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λcI mutants: Intragenic complementation and complementation with acI promoter mutant

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Summary

Complementation for the maintenance of lysogeny was studied by superinfecting λcIts lysogens at 34° C, and then heating to 43° C. With certain exceptions,ts mutants with defects in the left half of the repressor complementedts mutants with defects in the right half to produce a less heat-labile repressor (Fig. 3). AllcIamber mutants failed to complementcIts mutants. ThecI mutantc50 complements allts mutants. Mutations in Pre (cy) or genescII andcIII do not significantly affect the expression ofcI by a superinfecting λ genome in an immune lysogen. Mutants with very heat-labile repressors failed to complement λcy42 for the establishment of lysogeny at elevated temperatures, while those with less heatsensitive repressors apparently did complementcy.

According to a suggested model, the left side of thecI product is concerned primarily with subunit aggregation, while operator binding is the function of the right side of the oligomer.

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  1. Department of Microbiology, University of Southern California School of Medicine, 90033, Los Angeles, California, USA

    M. Lieb

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  1. M. Lieb
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Communicated by G. Bertani

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Lieb, M. λcI mutants: Intragenic complementation and complementation with acI promoter mutant. Molec. Gen. Genet. 146, 291–297 (1976). https://doi.org/10.1007/BF00701253

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