Summary
Fragments ofEscherichia coli DNA carrying genes for β-galactosidase, or for biosynthesis of guanine or biotin were recombined in vitro with λdv DNA. The cloned recombinant molecules recovered from transformedE. coli cells consisted of a biologically functional bacterial DNA fragment and, except for λdv-bio30-7, two λdv monomer units: one of the λdv units was used as the insertion site for the bacterial DNA, whereas the other was intact, and seemed to be responsible for the replication of the recombinant plasmid. The process which gives rise to these recombinant molecules at high frequency from mixtures of monomeric λdv DNA's and bacterial DNA fragments is discussed.
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Mukai, T., Matsubara, K. & Takagi, Y. Cloning bacterial genes with plasmid λdv. Molec. Gen. Genet. 146, 269–274 (1976). https://doi.org/10.1007/BF00701250
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DOI: https://doi.org/10.1007/BF00701250