The chirality breaking in the photolysis of leucine enantiomers and 5′CMP
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The inhibition of the photohydrate formation of the cytidine 5′ monophosphate (5′ CMP) under UV irradiation in the presence of the D-, L-leucine in phosphate aqueous solution (pH 7) has been studied. L-leucine demonstrates more effective in preventing 5′ CMP from photohydration than that of D-leucine. This photolysis method can be used instead of NMR spectroscopy to research weak nucleotide-amino acid interaction in aqueous solution especially for nonaromatic acid. A statistical thermodynamic stacking model is proposed to describe the mechanism producing chiral symmetry breaking. The calculated weak interaction energy ε is 1.16kT and 2.05kT for D-, and L-leucine respectively.
Key wordsChirality breaking leucine 5′ CMP weak interaction
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- 1.Eckhardt, C.J., Peachey, N.M., Swanson, D.R.T.,et al.: Separation of chiral phases in monolayer crystals of racemic amphiphiles,Nature 362 (1993), 614–616.Google Scholar
- 2.Schwartz, D.K.: Instant pattern in thin films,Nature 362 (1993), 593–594.Google Scholar
- 3.Chela-Flores, J.: The origin of chirality in protein amino acids,Chirality 6 (1994), 165–168.Google Scholar
- 4.Mclaren, A.D. and Shugar, D.:Photochemistry of Proteins and Nucleic Acids, Pergamon Press, (1994), pp. 88–107, 163–216.Google Scholar
- 5.Wang, S.Y.:Photochemistry and Photobiology of Nucleic Acids, Vol. 1, Chemistry, Academic Press, (1976), pp. 169–378.Google Scholar
- 6.Selinger, J.V., Wang, Z.G., Bruinsma, R.F.et al.:Chiral symmetry breaking in langmuir monolayers and smectic films, Phys. Rev. Lett. 70 (1993), 1139–1142.Google Scholar