Skip to main content
SpringerLink
Log in

Structural and functional analysis of T7D promoter and its complex withE. coli RNA polymerase

  • Published:
Russian Chemical Bulletin Aims and scope

Abstract

The DNA fragment containing D promoter of the T7 bacteriophage and its complex with DNA-dependent RNA polymerase undergo a conformational transition at 30 °C, which is accompanied by an increase in the rate of phosphodiester bond synthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. Rosenberg, T. R. Kadesch, and M. J. Chamberlin,J. Mol. Biol., 1982,155, 31.

    Google Scholar 

  2. J. H. Roe, R. R. Burgess, and M. T. Record, Jr.,J. Mol. Biol., 1985,184, 441.

    Google Scholar 

  3. J. E. Stefano and J. D. Gralla,J. Biol. Chem., 1980,255, 10423.

    Google Scholar 

  4. H. Buc and W. R. Mulligan,Biochemistry, 1985,24, 2712.

    Google Scholar 

  5. G. Duval-Valentin and R. Ehrlich,Nucl. Acids Res., 1986,14, 1967.

    Google Scholar 

  6. B. Hofer, D. Muller, and H. Koster,Nucl. Acids Res., 1985,13, 5995.

    Google Scholar 

  7. D. W. Cowing, J. Mescas, M. Record, and C. A. Gross,J. Mol. Biol., 1989,210, 521.

    Google Scholar 

  8. D. C. Straney and D. M. Crothers,Cell, 1985,43, 449.

    Google Scholar 

  9. T. R. Kadesch, S. Rosenberg, and M. J. Chamberlin,J. Mol. Biol., 1982,155, 1.

    Google Scholar 

  10. O. N. Ozoline, T. A. Uteshev, I. S. Masulis, and S. G. Kamzolova,Biochim. Biophys. Acta, 1993,1172, 251.

    Google Scholar 

  11. R. R. Burgess and J. J. Jendrisak,Biochemistry, 1975,14, 4634.

    Google Scholar 

  12. A. A. Maxam and W. Gilbert,Methods Enzymol.,65, 499.

  13. E. Bertrand-Burgraff, J. F. Lefevre, and M. Daune,Nucl. Acids Res., 1984,12, 1697.

    Google Scholar 

  14. Y.-T. Yu and J. L. Manley,Cell, 1986,45, 743.

    Google Scholar 

  15. N. Fujita and A. Ishihama,Molec. Gener. Genet., 1987,210, 10.

    Google Scholar 

  16. G. Yagil,Crit. Rev. Biochem. Mol. Biol.,26, 475.

  17. D. Suck, A. Lahm, and C. Oefner,Nature, 1988,332, 464.

    Google Scholar 

  18. H. Kanazawa, K. Mabuchi, and M. Futai,Biochem. Biophys. Res. Commun., 1982,107, 568.

    Google Scholar 

  19. R. L. Gourse,Nucl. Acids Res., 1988,16, 9789.

    Google Scholar 

  20. J. T. Newlands, W. Ross, K. Gosink, and R. L. Gourse,J. Mol. Biol., 1991,220, 569.

    Google Scholar 

  21. A. Spassky, K. Kirkegaard, and H. Buc,Biochemistry, 1985,24, 2723.

    Google Scholar 

  22. Y.-B. Shi, H. Gamper, B. V. Houten, and J. E. Hearst,J. Mol. Biol., 1988,199, 277.

    Google Scholar 

  23. C. Jeppesen, O. Buchardt, U. Henriksen, and P. Nielsen,Nucl. Acids Res., 1988,16, 5755.

    Google Scholar 

  24. M. E. Mulligan, D. F. Hawley, R. Entriken, and W. R. McClure,Nucl. Acids Res., 1980,12, 789.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, 142292, Pushchino Moscow region, Russian Federation

    O. N. Ozoline, I. S. Masulis, V. V. Chasov, N. N. Demina & S. G. Kamzolova

Authors
  1. O. N. Ozoline
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. S. Masulis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. V. Chasov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. N. Demina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. G. Kamzolova
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 7, pp. 1339–1374, July, 1995.

The present work was supported by the International Science Foundation (project No. RMY300) and the Russian Foundation for Basic Research (project No. 94-04-11306a).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ozoline, O.N., Masulis, I.S., Chasov, V.V. et al. Structural and functional analysis of T7D promoter and its complex withE. coli RNA polymerase. Russ Chem Bull 44, 1321–1326 (1995). https://doi.org/10.1007/BF00700912

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00700912

Key words

Search

Navigation