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Isolation of a dimethylsulfide-utilizingHyphomicrobium species and its application in biofiltration of polluted air

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Abstract

The methylotrophic bacteriumHyphomicrobium VS was enriched and isolated, using activated sewage sludge as inoculum in mineral medium containing dimethylsulfide (DMS) at a low concentration to prevent toxicity. DMS concentrations above 1 mM proved to be growth inhibiting.Hyphomicrobium VS could use DMS, dimethylsulfoxide (DMSO), methanol, formaldehyde, formate, and methylated amines as carbon and energy source. Carbon was assimilated via the serine pathway. DMS-grown cells respired sulfide, thiosulfate, methanethiol, dimethyldisulfide and dimethyltrisulfide.

To testHyphomicrobium VS for application in biofiltration of air polluted with volatile sulfur compounds two laboratory scale trickling biofilters with polyurethane and lava stone as carrier material were started up by inoculation with this bacterium. Both methanol- and DMS-grown cells could be used. Only a short adaptation period was needed. Short term experiments showed that high concentrations of DMS (1–2 µmol 1−1) were removed very efficiently by the biofilters at space velocities up to 100 h−1.

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Abbreviations

VSC:

volatile sulfur compounds

DMS:

dimethylsulfide

DMDS:

dimethyldisulfide

DMTS:

dimethyltrisulfide

MT:

methanethiol

DMSO:

dimethylsulfoxide

References

  • Blackmore MA & Quayle JR (1970) Microbial growth on oxalate by a route not involving glyoxylate carboligase. Biochem. J. 118: 53–59

    Google Scholar 

  • Buisman CJN, Geraats BG, IJspeert P & Lettinga G (1990) Optimization of sulphur production in a biotechnological sulphide-removing reactor. Biotechnol. Bioeng. 35: 50–56

    Google Scholar 

  • Charlson RJ, Lovelock JE, Andreae MO & Warren SG (1987) Oceanic phytoplankton sulphur, cloud albedo and climate. Nature 326: 655–661

    Google Scholar 

  • Cho KS, Hirai M & Shoda M (1991a) Degradation characteristics of hydrogen sulfide, methanethiol, dimethyl sulfide and dimethyl disulfide byThiobacillus thioparus DW44 isolated from peat biofilter. J. Ferment. Bioeng. 71: 384–389

    Google Scholar 

  • Cho KS, Hirai M & Shoda M (1992a) Enhanced removal efficiency of malodorous gases in a pilot-scale peat biofilter inoculated withThiobacillus thioparus DW44. J. Ferment. Bioeng. 73: 46–50

    Google Scholar 

  • Cho KS, Hirai M & Shoda M (1992b) Enhanced removability of odorous sulfur-containing gases by mixed cultures of purified bacteria from peat biofilters. J. Ferment. Bioeng. 73: 219–224

    Google Scholar 

  • Cho KS, Zhang L, Hirai M & Shoda M (1991b) Removal characteristics of hydrogen sulphide and methanethiol byThiobacillus sp. isolated from peat in biological deodorization. J. Ferment. Bioeng. 71: 44–49

    Google Scholar 

  • De Bont JAM, Van Dijken JP & Harder W (1981) Dimethyl sulphoxide and dimethyl sulphide as a carbon, sulphur and energy source for growth ofHyphomicrobium S. J. Gen. Microbiol. 127: 315–323

    Google Scholar 

  • Derikx PJL, Op den Camp HJM, Van der Drift C, Van Griensven LJLD & Vogels GD (1990) Odorous sulfur compounds emitted during production of compost used as a substrate in mushroom cultivation. Appl. Environ. Microbiol. 56: 176–180

    Google Scholar 

  • Derikx PJL, Simons FHM, Op den Camp HJM, Van der Drift C, Van Griensven LJLD & Vogels GD (1991) Evolution of volatile sulfur compounds during laboratory-scale incubations and indoor preparation of compost used as a substrate in mushroom cultivation. Appl. Environ. Microbiol. 57: 563–567

    Google Scholar 

  • Diks RMM (1992) The removal of dichloromethane from waste gases in a biological trickling filter. PhD Thesis. Technical University Eindhoven, The Netherlands

    Google Scholar 

  • Duine JA, Frank J & Westerling J (1978) Purification and properties of methanol dehydrogenase fromHyphomicrobium X. Biochim. Biophys. Acta 524: 277–287

    Google Scholar 

  • Harder W, Attwood MM & Quayle JR (1973) Methanol assimilation byHyphomicrobium sp. J. Gen. Microbiol. 78: 155–163

    Google Scholar 

  • Harder W & Attwood MM (1978) Biology, physiology and biochemistry of hyphomicrobia. Adv. Microb. Physiol. 17: 303–359

    Google Scholar 

  • Haywood GW & Large P (1981) Microbial oxidation of amines. Distribution, purification and properties of two primary amine oxidases from the yeastCandida boidinii grown on amines as sole nitrogen source. Biochem. J. 199: 187–201

    Google Scholar 

  • Kanagawa T & Kelly DP ((1986) Breakdown of DMS by mixed cultures and byThiobacillus thioparus. FEMS Microbiol. Lett. 34: 13–19

    Google Scholar 

  • Kelly DP & Smith NA (1990) Organic sulfur compounds in the environment. Biogeochemistry, microbiology and ecological aspects. Adv. Microb. Ecol. 11: 345–385

    Google Scholar 

  • Kiene RP (1988) Dimethylsulfide metabolism in salt marsh sediments. FEMS Microbiol. Ecol 53: 71–78

    Google Scholar 

  • Kirchner K, Hauk G & Rehm HJ (1987) Exhaust gas purification with immobilized monocultures (biocatalysts). Appl Microbiol. Biotechnol. 26: 579–587

    Google Scholar 

  • Sasahira T, Matsui K & Arai T (1991) Removal of hydrogen sulfide byHyphomicrobiumneptunium ATCC 15444. Chem. Pharm. Bull. 39: 2990–2993

    Google Scholar 

  • Sasahira T, Matsui K & Arai T (1992) Analysis of sulfur compounds produced with hydrogen sulfide byHyphomicrobium neptunium ATCC 15444. Chem. Pharm. Bull. 40: 799–801

    Google Scholar 

  • Shoda M (1991) Methods for the biological treatment of exhaust gases. In: Martin AM (Ed) Biological Degradation of Wastes (pp 31–46). Elsevier Science Publishers, London

    Google Scholar 

  • Suylen GMH (1988) Microbial metabolism of dimethyl sulphide and related compounds. PhD Thesis, Technical University Delft, The Netherlands

    Google Scholar 

  • Suylen GMH & Kuenen JG (1986) Chemostat enrichment and isolation ofHyphomicrobium EG, a dimethyl-sulphide oxidizing methylotroph and reevaluation ofThiobacillus MS1. Antonie van Leeuwenhoek 52: 281–293

    Google Scholar 

  • Suylen GMH, Stefess GC & Kuenen JG (1986) Chemolithotrophic potential of aHyphomicrobium species, capable of growth on methylated sulphur compounds. Arch. Microbiol. 146: 192–198

    Google Scholar 

  • Tanji Y, Kanagawa T & Mikami E (1989) Removal of dimethyl sulfide, methyl mercaptan, and hydrogen sulfide by immobilizedThiobacillus thioparus TK-m. J. Ferment. Bioeng. 67: 280–285

    Google Scholar 

  • Teunissen MJ, Marras SAE, Op den Camp HJM & Vogels GD (1989) Improved method for simultaneous determination of alcohols, volatile fatty acids, lactic acid or 2,3-butanediol in biological samples. J. Microbiol. Meth. 10: 247–254

    Google Scholar 

  • Tiwaree RS, Cho KS, Hirai M & Shoda M (1992) Biological deodorization of dimethyl sulfide using different fabrics as the carriers of microorganisms. Appl. Biochem. Biotechnol. 32: 135–148

    Google Scholar 

  • Van Alebeek GJWM, Hermans JMH, Keltjens JT, Vogels GD (1992) Quantification of intermediates involved in the cyclic 2,3-diphosphoglycerate metabolism of methanogenic bacteria by ionexchange chromatography. J. Chromatogr. 606: 65–71

    Google Scholar 

  • Zhang L, Hirai M & Shoda M (1991) Removal characteristics of dimethyl sulfide, methanethiol and hydrogen sulfide byHyphomicrobium sp. 155 isolated from peat biofilter. J. Ferment. Bioeng. 72: 392–396

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology & Evolutionary Biology, Faculty of Science, University of Nijmegen, Toernooiveld 1, NL-6525 ED, Nijmegen, The Netherlands

    Arjan Pol, Huub J. M. Op den Camp, Stefan G. M. Mees, Monique A. S. H. Kersten & Chris van der Drift

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  1. Arjan Pol
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  2. Huub J. M. Op den Camp
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  3. Stefan G. M. Mees
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  4. Monique A. S. H. Kersten
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  5. Chris van der Drift
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Pol, A., Op den Camp, H.J.M., Mees, S.G.M. et al. Isolation of a dimethylsulfide-utilizingHyphomicrobium species and its application in biofiltration of polluted air. Biodegradation 5, 105–112 (1994). https://doi.org/10.1007/BF00700635

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  • DOI: https://doi.org/10.1007/BF00700635

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