Journal of Biological Physics

, Volume 19, Issue 3, pp 155–165 | Cite as

Influx of Ca ion in cultured excitable cells studied by fluorescence microscope imaging technique

  • S. Takashima
  • T. Yoshida
  • Y. Takahashi
Article
  • 33 Downloads

Abstract

The spatial distribution and temporal variation of intracellular Ca ion in differentiated Neuroblastoma-Glia Hybridoma 108–15 cells (NG108–15) were investigated using a fluorescence microscope imaging technique. Fura-2 was used as a probe. Electrical current pulses of 10–20 µA were applied to axons connecting to NG cells in order to elicit the influx of Ca ion. The concentration of intracellular Ca is usually 50–80 nM in NG cells in the resting state. Upon stimulation, the Ca level increases by a factor of 2–5. The entry of Ca++ across cell membranes is followed by intracellular diffusion and the propagation of a wave front is clearly seen in digital images. The diffusion constant was calculated to be approximately 1.66×10−6 cm2/sec. This value is about one-fifth of the free diffusion coefficient of Ca ion in aqueous solution (7.82 × 10−6 cm2/sec). Cd ion, at the concentration of 1–2 mM, blocks the influx of Ca as expected whereas the influx is unaffected by TTX at the concentration of 0.1 – 0.2µM.

Key words

Intracellular Ca ion fluorescence microscope influx Neurobalstoma-Glia Hybridoma 108–15 cells 

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • S. Takashima
    • 1
  • T. Yoshida
    • 1
  • Y. Takahashi
    • 1
  1. 1.Department of BioengineeringUniversity of PennsylvaniaPhiladelphiaUSA

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