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Acta Neuropathologica

, Volume 26, Issue 2, pp 107–114 | Cite as

Glial changesin vitro induced by the inhibitor of succinic dehydrogenase

  • K. Renkawek
  • Z. Kraśnicka
  • T. Majdecki
  • M. J. Mossakowski
Original Investigations
  • 12 Downloads

Summary

The object of the experiment was to obtainin vitro the glial changes typical of hepatogenic encephalopathy, after inhibition of one of the stages of the Krebs cycle. A chemical inhibitor of succinic dehydrogenase, sodium malonate, was added to the culture medium in various concentrations. The experiment was carried out in four groups. The damage to the metabolism of the Krebs cycle resulted in the appearance of typical Opalski cells and intermediate cell forms. These pathological cells exhibited the same morphological and histochemical properties as those observed in experimental hepatogenic encephalopathy in vivo and in vitro. The striking feature of the Opalski cells was a significant decrease of SDH and GDH activity.

The authors discuss the possible pathogenesis of the glial changes which are probably due to the disturbance of ammonia detoxication.

Key words

Experimental Glial Changes Opalski Cells Tissue Culture SDH Inhibition 

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • K. Renkawek
    • 1
  • Z. Kraśnicka
    • 1
  • T. Majdecki
    • 1
  • M. J. Mossakowski
    • 1
  1. 1.Department of Neuropathology of Experimental and Clinical Medical Research CentrePolish Academy of SciencesWarfzawaPoland

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