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Structural comparison of I-A antigens produced by a cloned murine T suppressor cell line with B-Cell-Derived I-A

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Abstract

A cloned, antigen-specific T suppressor cell line derived from a CBA mouse expresses large amounts of I-A and I-E antigens. Comparative two-dimensional polyacrylamid gel electrophoresis of biosynthetically labeled I-A antigens immunoprecipitated with a variety of monoclonal I-Ak-specific antibodies suggested that α,β and Ii polypeptide chains are identical with B-cell-derived I-A. Dimeric complexes formed by I-A chains derived from B or T suppressor cells were also similar with two major exceptions. Pulse-labeled T-cell-derived la antigen was complexed with two additional unknown components of about 31K. These components were not visible in pulse-chased (processed) materials. In addition, T suppressor-cell-derived I-A antigens did not contain S-S linked dimers consisting of processed α andβ chains, which are usually formed during solubilization of B cells. We consider the possibility that in T cells these chains are associated with other structures, thus preventing S-S linkage between α andβ chains.

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Abbreviations

BSA:

bovine serum albumin

Ii chain:

invariant chain

LPS:

lipopolysaccharide

mc:

monoclonal

mAb:

monoclonal antibody

TCGF:

T cell growth factor

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Authors and Affiliations

  1. German Cancer Research Center, Institute of Immunology and Genetics, DKFZ, Im Neuenheimer Feld 280, D-6900, Heidelberg, Germany

    Norbert Kocht, Bernd Arnold & Günter J. Hämmerling

  2. Department of Immunology, University of Münster, D-4400, Münster, Germany

    Joachim Heuer & Eckehart Kölsch

Authors
  1. Norbert Kocht
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  2. Bernd Arnold
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  3. Günter J. Hämmerling
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  4. Joachim Heuer
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  5. Eckehart Kölsch
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Dedicated to Professor O. Westphal on the occasion of his seventieth birthday.

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Kocht, N., Arnold, B., Hämmerling, G.J. et al. Structural comparison of I-A antigens produced by a cloned murine T suppressor cell line with B-Cell-Derived I-A. Immunogenetics 17, 497–505 (1983). https://doi.org/10.1007/BF00696873

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  • DOI: https://doi.org/10.1007/BF00696873

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