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Immunogenetics

, Volume 17, Issue 5, pp 445–455 | Cite as

Genetic analysis of theT-H-2 region in non-t Chromosomes

I. Two new congenic strains isolateQglo-1 fromH-2
  • Eugene M. Rinchik
  • D. Bernard Amos
Original Investigations
  • 16 Downloads

Abstract

A general breeding protocol useful in the construction of congenic lines of mice disparate in the 15 cMT-H-2 region of chromosome 17 in non-t chromosomes is described. Two such congenic lines, B6.TC2/Rn and B6.TC3/Rn, were derived from the C57BL/6J and B6.C-H-2d/By strains using this protocol. Both B6.TC2 and B6.TC3 dissociate the quantitative activity locus for glyoxalase I (Qglo-1) from theH-2 complex, and hence possess BALB/cBy DNA centromeric toH-2. However, neither new strain is able to map anyH-2-associated restriction fragment length polymorphism with anH-2 cDNA probe even though both strains are recombinant in the 2 cMQglo-1-H-2K interval.

Keywords

Genetic Analysis Fragment Length Length Polymorphism Restriction Fragment Length Polymorphism Restriction Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Eugene M. Rinchik
    • 1
  • D. Bernard Amos
    • 1
  1. 1.Department of Microbiology and ImmunologyDuke University Medical CenterDurham

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