, Volume 17, Issue 5, pp 445–455 | Cite as

Genetic analysis of theT-H-2 region in non-t Chromosomes

I. Two new congenic strains isolateQglo-1 fromH-2
  • Eugene M. Rinchik
  • D. Bernard Amos
Original Investigations


A general breeding protocol useful in the construction of congenic lines of mice disparate in the 15 cMT-H-2 region of chromosome 17 in non-t chromosomes is described. Two such congenic lines, B6.TC2/Rn and B6.TC3/Rn, were derived from the C57BL/6J and B6.C-H-2d/By strains using this protocol. Both B6.TC2 and B6.TC3 dissociate the quantitative activity locus for glyoxalase I (Qglo-1) from theH-2 complex, and hence possess BALB/cBy DNA centromeric toH-2. However, neither new strain is able to map anyH-2-associated restriction fragment length polymorphism with anH-2 cDNA probe even though both strains are recombinant in the 2 cMQglo-1-H-2K interval.


Genetic Analysis Fragment Length Length Polymorphism Restriction Fragment Length Polymorphism Restriction Fragment 
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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Eugene M. Rinchik
    • 1
  • D. Bernard Amos
    • 1
  1. 1.Department of Microbiology and ImmunologyDuke University Medical CenterDurham

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