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Acta Neuropathologica

, Volume 8, Issue 4, pp 321–330 | Cite as

The ultrastructure of a human glioblastoma multiforme-derived tumor heterologously transplanted to giunea pig eye and brain

  • Mary M. Herman
  • W. Robert Adams
  • Elias E. Manuelidis
Original Investigations

Summary

Studies with the light and the electron microscope were carried out on a human glioblastoma multiforme maintained for 17 years in heterologous growth in the guinea pig. Sublines of the tumor growing in the brain and in the eye were studied. With the light microscope the tumor had lost the morphological characteristics of glioblastoma and was composed almost entirely of spongioblasts as judged by metallic stains. The ultrastructure of the tumor cells was similar to that of astrocytes in astrocytomas as described by others. The elongated spindle-shaped cells contained a normal complement of the usual cytoplasmic organelles. They also contained prominent cytoplasmic fibrils, whose morphology suggested a repetitive structural subunit; and frequent cytoplasmic vacuoles and inclusions displaying considerable variation. The morphology of the tumor cells growing in the brain and eye was similar, but in the brain tumor the architecture was looser and there was a relative increase in extracellular space.

Keywords

Brain Tumor Fibril Astrocytoma Extracellular Space Relative Increase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Licht- und elektronenoptische Untersuchungen wurden an einem menschlichen Glioblastoma multiforme durchgeführt, das 17 Jahre hindurch am heterologen Wachstum im Meerschweinchen erhalten wurde. Unterarten des Tumors im Gehirn und Auge wurden untersucht. Lichtmikroskopisch hatte der Tumor die morphologischen Merkmale des Glioblastoms verloren und baute sich fast ausschließlich aus Spongioblasten auf, wie Silberfärbungen zeigten. Die Ultrastruktur der Tumorzellen ähnelte der von Astrocyten, wie sie in Astrocytomen beschrieben wurden. Die langgestreckten, spindelförmigen Zellen zeigten einen normalen Aufbau der üblichen Zellorganellen. Sie enthielten auch reichlich hervortretende cytoplasmatische Fibrillen, deren Morphologie eine repetitive strukturelle Untergruppierung vermuten lassen, sowie häufige cytoplasmatische Vacuolen und Einschlüsse von erheblicher Variabilität. Die Morphologie der im Gehirn und im Auge wachsenden Tumorzellen war ähnlich, doch erwies sich die Architektur im Gehirntumor als lockerer und zeigte eine relative Zunahme des extracellulären Raumes.

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Copyright information

© Springer-Verlag 1967

Authors and Affiliations

  • Mary M. Herman
    • 1
    • 2
  • W. Robert Adams
    • 1
    • 2
  • Elias E. Manuelidis
    • 1
    • 2
  1. 1.The Department of PathologyYale University, School of MedicineNew HavenUSA
  2. 2.The Veteran's Administration HospitalWest Haven

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