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Acta Neuropathologica

, Volume 32, Issue 3, pp 257–267 | Cite as

Plasma membrane structures of medulloblastoma and cerebellar sarcoma

  • Eiichi Tani
  • Tatsuo Morimura
  • Keizo Kaba
  • Noboru Higashi
Article

Summary

Three medulloblastomas and 1 cerebellar sarcoma were studied on their plasma membrane structures. The average number of membrane particles per μm2 plasma membrane was 710 on face A and 70 on face B of medulloblastoma and 1280 on face A and 160 on face B of cerebellar sarcoma. The membrane particles were often aggregated in medulloblastoma and diffusely scattered in cerebellar sarcoma. Small gap junctions were occasionally found in cerebellar sarcoma and not evident in medulloblastoma. Round membrane protrusions, about 0.5–0.6 μ in diameter and provided with several small depressions on their foot, were often observed in region of narrow perinuclear cytoplasm of cerebellar sarcoma and different in structure from cytoplasmic processes. The present series is too limited in number to allow a definite conclusion, but indicates that the plasma membrane structures are different in medulloblastoma and cerebellar sarcoma.

Key words

Medulloblastoma Cerebellar Sarcoma Plasma Membrane Membrane Particles Freeze-Fracture 

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References

  1. Bischoff, A., Moor, H.: Ultrastructural differences between the myelin sheaths of peripheral nerve fibers and CNS white matter. Z. Zellforsch.81, 303–310 (1967a)Google Scholar
  2. Bischoff, A., Moor, H.: The ultrastructure of the “difference factor” in the myelin. Z. Zellforsch.81, 571–580 (1967b)Google Scholar
  3. Branton, D.: Fracture faces of frozen membranes. Proc. nat. Acad. Sci. (Wash.)55, 1048–1056 (1966)Google Scholar
  4. Branton, D.: Fracture faces of frozen myelin. Exp. Cell Res.45, 703–707 (1967)Google Scholar
  5. Branton, D.: Freeze-etching studies of membrane structure. Phil. Trans.261, 133–138 (1971)Google Scholar
  6. Branton, D., Elgsaeter, A., James, R.: Freze-etch studies of membrane organization and reorganization. Fed. europ. Biochem. Soc. Proc. Meet.28, 165–172 (1972)Google Scholar
  7. Bretscher, M. S.: A major protein which spans the human erythrocyte membrane. J. molec. Biol.59, 351–357 (1971)Google Scholar
  8. Cervós-Navarro, J., Matakas, F.: La contribution de la microscopie électronique à l'origine tissulaire des médulloblastomes. Neurochirurgie16, 551–557 (1970)Google Scholar
  9. Deamer, D. W., Leonard, R., Tardieu, A., Branton, D.: Lamellar and hexagonal lipid phase visualized by freeze-etching. Biochim. biophys. Acta (Amst.)219, 47–60 (1970)Google Scholar
  10. Elgsaeter, A., Shotton, D., Branton, D.: Control of protein distribution in the erythrocyte membrane. J. Cell. Biol.59, 89a (1973)Google Scholar
  11. Escourolle, R., Poirier, J.: L'ultrastructure du médulloblastome cérébeleux. Ann. Anat. path.12, 121–136 (1967)Google Scholar
  12. Foerster, O., Gagel, O.: Das umschriebene Arachnoidealsarkom des Kleinhirms. Z. ges. Neurol. Psychiat.164, 565–580 (1939)Google Scholar
  13. Gullotta, F., Kersting, G.: The ultrastructure of medulloblastoma in tissue culture. Virchows Arch. path. Anat.356, 111–118 (1972)Google Scholar
  14. Hong, K., Hubbell, W. L.: Preparation and properties of phospholipid bilayers containing rhodopsin. Proc. nat. Acad. Sci. (Wash.)69, 2617–2621 (1972)Google Scholar
  15. Kadin, M. E., Rubinstein, L. J., Nelson, J. S.: Neonatal cerebellar medulloblastoma originating from the fetal external granular layer. J. Neuropath. exp. Neurol.29, 583–600 (1970).Google Scholar
  16. Kernohan, J. W., Uihlein, A.: Sarcoma of the Brain, Springfield Ill.: Ch. C. Thomas 1962Google Scholar
  17. Matakas, F., Cervós-Navarro, J., Gullotta, F.: The ultrastructure of medulloblastomas. Acta neuropath. (Berl.)16, 271–284 (1970)Google Scholar
  18. McNutt, N. S.: Freeze-etch study of plasma membrane differentiation and junctions in a kreatinized stratified squamous epithelium. J. Cell Biol.59, 210a (1973)Google Scholar
  19. Pinto Da Silva, P.: Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation. J. Cell Biol.53, 777–787 (1972)Google Scholar
  20. Pinto Da Silva, P., Branton, D.: Membrane splitting in freeze-etching: covalently bound ferritin as a membrane marker. J. Cell Biol.45, 598–605 (1970)Google Scholar
  21. Pinto Da Silva, P., Douglas, S. D., Branton, D.: Localization of A antigen sites on human erythrocyte ghosts. Nature (Lond.)232, 194–196 (1971)Google Scholar
  22. Poon, T. P., Hirono, A., Zimmerman, H. M.: Electron microscopic atlas of brain tumors. New York-London: Grune and Stratton 1971Google Scholar
  23. Ramsey, H. J., Kernohan, J. W.: Circumscribed sarcoma of the cerebellum. An electron microscopic study. J. Neuropyth. exp. Neurol.23, 706–718 (1974)Google Scholar
  24. Rubinstein, L. J., Northfield, D. W. C.: The medulloblastoma and the so-called “arachnoidal cerebellar sarcoma”. Brain87, 379–412 (1964)Google Scholar
  25. Singer, S. J.: The molecular organization of biological membranes. L. I. Rothfield, ed., pp. 145–222. New York: Academic Press 1971Google Scholar
  26. Steck, T. L., Fairbanks, G., Wallach, D. F. H.: Disposition of the major proteins in the isolated erythrocyte membrane. Proteolytic dissection. Biochemistry10, 2617–2624 (1971)Google Scholar
  27. Tani, E., Higashi, N.: Freeze-fracture study of human brain tumors. Child's Brain1, 46–71 (1975)Google Scholar
  28. Tani, E., Ikeda, K., Nishiura, M.: Freeze-etching images of central myelinated nerve fibers. J. Neurocytol.2, 305–314 (1973)Google Scholar
  29. Tani, E., Ikeda, K., Nishiura, M., Higashi, N.: Specialized intercellular junctions and ciliary necklace in rat brain. Cell Tiss. Res.151, 57–68 (1974a)Google Scholar
  30. Tani, E., Ikeda, K., Yamagata, S., Nishiura, M., Higashi, N.: Specialized junctional complexes in human meningioma. Acta neuropath. (Berl.)28, 305–315 (1974b)Google Scholar
  31. Tani, E., Nishiura, M., Higashi, N.: Freeze-fracture studies of gap junctions in human meningioma. Acta neuropath. (Berl.)30, 305–314 (1974c)Google Scholar
  32. Tani, E., Takeuchi, J., Ishijima, Y., Higashi, N., Fuijihara, E., Ametani, T., Ando, K.: Elongated nuclear sheet and intranuclear myelin figure of human medulloblastoma. Cancer Res.31, 2120–2129 (1971)Google Scholar
  33. Tani, E.: unpublished observationGoogle Scholar
  34. Tillack, T. W., Marechesi, V. T.: Demonstration of the outer surface of freeze-etched red blood cell membranes. J. Cell Biol.45, 649–653 (1970)Google Scholar
  35. Tillack, T. W., Scott, R. E., Marchesi, V. T.: The structure of erythrocyte membranes studied by freeze etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles. J. exp. Med.135, 1209–1227 (1972)Google Scholar
  36. Voigt, W. H.: Elektronenmikroskopische Beobachtungen an menschlichen Medulloblastomen. Dtsch. Z. Nervenheilk.192, 290–309 (1968)Google Scholar
  37. Waga, S.: A histological and electron microscopic study on medulloblastomas and cerebellar sarcomas. Arch. Jap. Chir.34, 436–454 (1965)Google Scholar
  38. Zimmerman, H. M., Netsky, M. G., Davidoff, L. M.: Atlas of Tumors of the Nervous System, p. 103. Philadelphia: Lea and Febriger 1956Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Eiichi Tani
    • 1
    • 2
  • Tatsuo Morimura
    • 1
    • 2
  • Keizo Kaba
    • 1
    • 2
  • Noboru Higashi
    • 1
    • 2
  1. 1.Department of NeurosurgeryHyogo College of MedicineHyogoJapan
  2. 2.Institute for Virus ResearchKyoto UniversityKyotoJapan

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