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Acta Neuropathologica

, Volume 64, Issue 2, pp 108–113 | Cite as

The use of semiautomatic morphometry in the study of normal rat gastrocnemius muscle fibers

  • W. De Coster
  • J. De Reuck
  • H. Vander Eecken
Original Works

Summary

Based on a fixed number of EM photomicrographs a semiautomatic image analysis is made of normal gastrocnemius muscle fibers to obtain reference values for comparison with pathologic data. Z disc, mitochondria, tubular system, and sarcoplasmic reticulum are measured. It is stated that no definite fiber type differentiation is possible based on these quantitative data. However, measurements of Z disc and mitochondria are suggestive of such a differentiation. On the other hand, all data reach a Gaussian distribution on a logarithmic scale after a 5% correction factor is introduced.

Key words

Image analysis Electron microscopy Muscle fibers 

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References

  1. De Coster W, De Reuck J, Sieben G, Vander Eecken H (1981) Early ultrastructural changes in aging rat gastrocnemius muscle: a stereologic study. Muscle Nerve 4:111–116Google Scholar
  2. Eisenberg BR, Kuda AM, Peter JB (1974) Stereological analysis of mammalian skeletal muscle. I. Soleus muscle of the adult guinea pig. J Cell Biol 60:732–754Google Scholar
  3. Eisenberg BR, Kuda AM (1975) Stereological analysis of mammalian skeletal muscle. II. White vastus muscle of the adult guinea pig. J Ultrastruct Res 51:176–187Google Scholar
  4. Rigaut JP, Berggren P, Robertson B (1983) Stereological study of lung with IBAS image analyser. J Microse 130:53–61Google Scholar
  5. Salmons S, Gale DR, Sreter FA (1978) Ultrastructural aspects of the transformation of muscle fibre type by longterm stimulation: changes in Z discs and mitochondria. J Anat 127:17–31Google Scholar
  6. Sjöström M, Ängquist KA, Bylund AC, Friden J, Gustavsson L, Schersten T (1982) Morphometric analysis of human muscle fiber types. Muscle Nerve 5:538–553Google Scholar
  7. Weibel ER (1969) Stereological principles for morphometry in electron-microscopic cytology. Int Rev Cytol 26:235–302Google Scholar
  8. Weibel ER (1973) A stereological method for estimating volume and surface of sarcoplasmic reticulum. J Microsc 95:229–242Google Scholar
  9. Weibel ER (1979) Stereological methods, vols 1 and 2. Academic Press, LondonGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • W. De Coster
    • 1
  • J. De Reuck
    • 1
  • H. Vander Eecken
    • 1
  1. 1.Neuropathology Unit, Dept, of NeurologyState UniversityGhentBelgium

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