The use of semiautomatic morphometry in the study of normal rat gastrocnemius muscle fibers
- 18 Downloads
Based on a fixed number of EM photomicrographs a semiautomatic image analysis is made of normal gastrocnemius muscle fibers to obtain reference values for comparison with pathologic data. Z disc, mitochondria, tubular system, and sarcoplasmic reticulum are measured. It is stated that no definite fiber type differentiation is possible based on these quantitative data. However, measurements of Z disc and mitochondria are suggestive of such a differentiation. On the other hand, all data reach a Gaussian distribution on a logarithmic scale after a 5% correction factor is introduced.
Key wordsImage analysis Electron microscopy Muscle fibers
Unable to display preview. Download preview PDF.
- De Coster W, De Reuck J, Sieben G, Vander Eecken H (1981) Early ultrastructural changes in aging rat gastrocnemius muscle: a stereologic study. Muscle Nerve 4:111–116Google Scholar
- Eisenberg BR, Kuda AM, Peter JB (1974) Stereological analysis of mammalian skeletal muscle. I. Soleus muscle of the adult guinea pig. J Cell Biol 60:732–754Google Scholar
- Eisenberg BR, Kuda AM (1975) Stereological analysis of mammalian skeletal muscle. II. White vastus muscle of the adult guinea pig. J Ultrastruct Res 51:176–187Google Scholar
- Rigaut JP, Berggren P, Robertson B (1983) Stereological study of lung with IBAS image analyser. J Microse 130:53–61Google Scholar
- Salmons S, Gale DR, Sreter FA (1978) Ultrastructural aspects of the transformation of muscle fibre type by longterm stimulation: changes in Z discs and mitochondria. J Anat 127:17–31Google Scholar
- Sjöström M, Ängquist KA, Bylund AC, Friden J, Gustavsson L, Schersten T (1982) Morphometric analysis of human muscle fiber types. Muscle Nerve 5:538–553Google Scholar
- Weibel ER (1969) Stereological principles for morphometry in electron-microscopic cytology. Int Rev Cytol 26:235–302Google Scholar
- Weibel ER (1973) A stereological method for estimating volume and surface of sarcoplasmic reticulum. J Microsc 95:229–242Google Scholar
- Weibel ER (1979) Stereological methods, vols 1 and 2. Academic Press, LondonGoogle Scholar