Virchows Archiv A

, Volume 404, Issue 4, pp 369–380 | Cite as

Immunoelectronmicroscopic study on the transport of secretory IgA in the lower respiratory tract and alveoli

  • Hajime Haimoto
  • Hiroshi Nagura
  • Munehisa Imaizumi
  • Keiichi Watanabe
  • Soichi Iijima
Article

Summary

To define the immunocytochemical localization of secretory component (SC), IgA and J chain in human bronchioles and alveoli, a direct peroxidase-labeled antibody method was used. SC was found in non-ciliated cells of the bronchioles including respiratory bronchioles and type II alveolar epithelial cells, whereas SC was rarely present in ciliated cells and type I alveolar epithelial cells and was absent from goblet cells. In the positively reacting cells, SC was found in secretory protein synthetic organelles such as perinuclear spaces and endoplasmic reticulum, Golgi complexes, and on the external surfaces of the apical and basolateral plasma membranes. IgA and J chain were localized in the epithelial cells where SC was found. Ultrastructually IgA was present on the apical and basolateral plasma membranes, in pinocytic invaginations of the membranes, and in vesicles distributed through the cytoplasm, especially in the apical cytoplasm of the epithelial cells where SC was found. In addition, IgA and J chain were found to be associated with the endothelial cells of the capillaries, plasma cells and the surrounding interstitium. These observations suggest that SC is synthesized and secreted by epithelial cells, especially non-ciliated cells of the bronchioles including respiratory bronchioles and type II alveolar epithelial cells. They also suggest that secretory IgA (sIgA) is transported into alveolar spaces and the bronchiolar lumen through these cells by SC-mediated transport mechanism. This sIgA may play an important role in defense mechanisms of the lower respiratory tract and alveoli.

Key words

IgA-Secretory Component J chain Lung Immunocytochemistry 

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References

  1. Bernaudin JF, Bellon B, Pinchon MC, Kuhn J, Druet P, Bignon J (1982) Permeability of the blood-air barrier to antiperoxidase antibodies and their fragments in the normal rat lung. Am Rev Respir Dis 125:734–739Google Scholar
  2. Brandtzaeg P (1973) Structure, synthesis and external transfer of mucosal immunoglobulins. Ann Immunol (Paris) 124:417–438Google Scholar
  3. Brandtzaeg P (1974) Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol fixation technique. Immunology 26:1101–1114Google Scholar
  4. Brandtzaeg P (1976a) Complex formation between secretory component and human immunoglobulins related to their content of J chain. Scand J Immunol 5:411–419Google Scholar
  5. Brandtzaeg P (1976b) Studies on J chain and binding site for secretory component in circulating human B cells. 1. The surface membrane. Clin Exp Immunol 25:50–58Google Scholar
  6. Brandtzaeg P (1977) Immunohistochemical studies on various aspects of glandular immunoglobulin transport in man. Histochem J 9:553–572Google Scholar
  7. Brown WR, Isobe Y, Nakane PK (1976) Studies on translocation of immunoglobulins across intestinal epithelium. II. Immunoelectronmicroscopic localization of immunoglobulins and secretory component in human intestinal mucosa. Gastroenterology 71:985–995Google Scholar
  8. Crago SS, Kulhavy R, Prince SJ, Mestecky J. (1978) Secretory component on epithelial cells is a surface receptor for polymeric immunoglobulins. J Exp Med 147:1832–1837Google Scholar
  9. Goodman MR, Link DW, Brown WR, Nakane PK (1981) Ultrastructural evidence of transport of secretory IgA (sIgA) across bronchial epithelium. Am Rev Respir Dis 123:115–119Google Scholar
  10. Hand WE, Cantey JR (1974) Antibacterial mechanisms of the lower respiratory tract. I. Immunoglobulin synthesis and secretion. J Clin Invest 53:354–362Google Scholar
  11. Kaltreider HB, Kyselka L, Salmon SE (1974) Immunology of the lower respiratory tract. II. The plaque-forming response of canine lymphoid tissues to sheep erythrocytes after intrapulmonary or intravenous immunization. J Clin Invest 54:263–270Google Scholar
  12. Kobayashi K, Vaerman JP, Heremans JF (1973) Improved procedure for the isolation of J-chain from human polymeric immunoglobulins. Biochim Biophys Acta 303:105–117Google Scholar
  13. Koshland ME (1975) Structure and function of the J chain. Adv Immunol 20:41–69Google Scholar
  14. Laurenzi GA, Potter RT, Kass EH (1961) Bacteriologic flora of the lower respiratory tract. New Eng J Med 265:1273–1278Google Scholar
  15. Lees AW, McNaught W (1959) Bacteriology of lower-respiratory-tract secretions, sputum, and upper-respiratory-tract secretions in normals and chronic bronchitics. Lancet 2 1112–1115Google Scholar
  16. Martinez-Tello FJ, Braun DG, Blanc WA (1968) Immunoglobulin production in bronchial mucosa and bronchial lymph nodes, particularly in cystic fibrosis of the pancreas. J Immunol 101:989–1003Google Scholar
  17. McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083Google Scholar
  18. Nakamura T, Nagura H, Watanabe K, Komatsu N, Uchikoshi S, Kobayashi K, Nabeshima J (1982) Immunocytochemical localization of secretory immunoglobulins in human parotid and submandibular glands. J Electron Microsc 31:151–161Google Scholar
  19. Nagura H, Brandtzaeg P, Nakane PK, Brown WR (1979a) Ultrastructural localization of J chain in human intestinal mucosa. J Immunol 123:1044–1050Google Scholar
  20. Nagura H, Nakane PK, Brown, WR (1979b) Translocation of dimeric IgA through neoplastic colon cells in vitro. J Immunol 123:2359–2368Google Scholar
  21. Nagura H, Smith PD, Nakane PK, Brown WR (1981) IgA in human bile and liver. J Immunol 126:587–595Google Scholar
  22. Nagura H, Tsutsumi Y, Hasegawa H, Watanabe K, Nakane PK, Brown WR (1983) IgA plasma cells in biliary mucosa: A likely source of locally synthesized IgA in human hepatic bile. Clin Exp Immunol 54:671–680Google Scholar
  23. Neuhouse M, Sanchis J, Bienenstock J (1976) Lung defence mechanisms. New Eng J Med 295:1045–1052Google Scholar
  24. Paciga JE, Shelley SA, Balis JU (1980) Secretory IgA is a component of rabbit lung surfactant. Biochim Biophys Acta 31:487–494Google Scholar
  25. Rossen RD, Morgan C, Hsu KC, Butler WT, Rose HM (1968) Localization of 11 s external secretory IgA by immunofluorescence in tissues lining the oral and respiratory passages in man. J Immunol 100:706–717Google Scholar
  26. Soutar CA (1976) Distribution of plasma cells and other cells containing immunoglobulin in the respiratory tract of normal man and class of immunoglobulin contained therein. Thorax 31:158–166Google Scholar
  27. Tomasi TB, Tan EM, Solomon A, Prendergast RA (1965) Characteristics of an immune system common to certain external secretions. J Exp Med 121:101–124Google Scholar
  28. Tomasi TB, Grey HM (1972) Structure and function of immunoglobulin A. Prog Allergy 16:81–213Google Scholar
  29. Wilson MB, Nakane PK (1978) In immunofluorescence and related staining techniques. Elsevier Excerpta Medica North-Holland, Amsterdam, p 215Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Hajime Haimoto
    • 1
  • Hiroshi Nagura
    • 2
  • Munehisa Imaizumi
    • 3
  • Keiichi Watanabe
    • 4
  • Soichi Iijima
    • 1
  1. 1.Department of PathologyNagoya University School of MedicineShowa, Nagoya
  2. 2.Laboratory of Germfree Life Research Institute for Disease Mechanism and ControlNagoya University School of MedicineShowa, Nagoya
  3. 3.Department of SurgeryNagoya University School of MedicineShowa, Nagoya
  4. 4.Department of PathologyTokai University School of MedicineJapan

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