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Abnormal binding of negatively charged serum proteins to diabetic basement membranes is largely a systemic phenomenon

Virchows Archiv A volume 405, pages 141–154 (1984)Cite this article

Summary

Direct immunofluorescence employing goat anti-human IgG, IgA, IgM, C3 component of complement, fibrinogen, albumin, and polyvalent immunoglobulins was performed on postmortem samples of gingiva, parotid gland, thyroid, kidney, and pancreas tissue of 15 diabetic and 15 control patients.

Basement membrane thickness quantification of kidney tubules, gingival capillaries, and parotid gland ducts and acini was also done utilizing a calibrated magnifier on uniformly enlarged photomicrographs which had been specially stained to highlight basement membranes.

Results revealed binding of IgG, albumin, and polyvalent immunoglobulin to kidney glomerular and tubular basement membranes and parotid ductal and acinar basement membranes in all diabetic subjects. Thyroid follicular basement membranes were positive in 8 of 15 diabetic patients for the same antisera. All gingival and pancreatic tissue from diabetic and control patients was negative for binding of all serum proteins tested.

Basement membrane thickening in kidney tubules and gingival capillaries was observed in diabetic subjects; however, there was no apparent difference between diabetic and control patients in thickness of ductal or acinar basement membranes of the parotid gland.

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Affiliations

  1. Department of Oral Pathology, University of Minnesota, 515 Delaware St. S.E, 55455, Minneapolis, MN

    Valerie Murrah & John Sauk

  2. Department of Pathology, Hennepin County Medical Center, 55455, Minneapolis, MN

    Valerie Murrah & John Crosson

Authors
  1. Valerie Murrah
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  2. John Crosson
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  3. John Sauk
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Murrah, V., Crosson, J. & Sauk, J. Abnormal binding of negatively charged serum proteins to diabetic basement membranes is largely a systemic phenomenon. Vichows Archiv A Pathol Anat 405, 141–154 (1984). https://doi.org/10.1007/BF00694932

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  • DOI: https://doi.org/10.1007/BF00694932

Key words

  • Diabetes mellitus
  • Basement membrane
  • Immunofluorescence