Summary
An immunoperoxidase technique has been utilized for the localization of carcinoembryonic antigen (CEA), Secretory component (SC) and lysozyme (LZ) in normal and cancerous common bile duct tissues. Little or no CEA was found in the non-cancerous common bile duct tissues. SC was found in the surface epithelium and accessory gland epithelium and LZ was demonstrated only in the accessory glands. Some inflammatory cells were also positively stained for LZ. In adenocarcinoma, CEA was always present on the luminar border of the carcinoma cells, occasionally with intercellular and intracellular localization. LZ was absent, or only faintly detected in carcinoma. SC was generally distributed in well-differentiated adenocarcinoma cells, but showed a reduced intensity of staining with progressive dedifferentiation. These findings suggest that CEA, SC and LZ could be useful markers providing valuable information in the pathological diagnosis of bile duct carcinoma.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahnen DJ, Nakane PK, Brown WR (1982) Ultrastructural localization of carcinoembryonic antigen in normal intestine and colon cancer. Abnormal distribution of CEA on the surface of colon cancer cell. Cancer 49:2077–2090
Albores-Saavedra J, Nadji M, Morales AR, Henson DE (1983) Carcinoembryonic antigen in normal, preneoplastic and neoplastic gallbladder epithelium. Cancer 52:1069–1072
Burtin P (1978) The carcinoembryonic antigen (CEA) of the digestive system and the antigens cross-reacting with it. Ann Immunol (Inst Pasteur) 129c:185–198
Crago SS, Kulhary R, Prince SJ, Mestecky J (1978) Secretory component on epithelial cells in a surface receptor for polymeric immunoglobulins. J Exp Med 147:1832–1837
Elias H, Sherrick JC (1968) Morphology of the Liver, Academic Press, New York, pp 137–185
Gold P, Freedmann SO (1965) Specific carcinoembryonic antigens of the human digestive system. J Exp Med 122:467–481
Holburn AM, Mach JP, MacDonald D, Newland M (1974) Studies of the association of the A, B and Lewis blood group antigens with carcinoembryonic antigen (CEA). Immunology 26:831–843
Isaacson P, LeVann HP (1976) The demonstration of carcinoembryonic antigen in colorectal carcinoma and colonic polyp using an immunoperoxidase technique. Cancer 38:1348–1356
Isaacson P (1982) Immunoperoxidase study of the secretory immunoglobulin system and lysozyme in normal and diseased gastric mucosa. Gut 23:578–588
Isobe Y, Chen ST, Nakane PK, Brown WR (1977) Studies on translocation of immunoglobulins across intestinal epithelium. I. Improvements in peroxidase-labeled antibody method for application to study of human intestinal mucosa. Acta Histochem Cytochem 10:177–183
Katsuyama T, Spicer SS (1978) Histochemical differentiation of complex carbohydrate with variants of the Concanavalin A-horseradish peroxidase method. J Histochem Cytochem 26:233–250
Klockards M, Reitamo S (1975) Tissue distribution of lysozyme in man. J Histochem Cytochem 23:932–940
Mason DY, Taylor CR (1975) The distribution of muramidase (lysozyme) in human tissues. J Clin Pathol 28:124–132
McMinn RMH, Kugler JH (1961) The glands of the bile and pancreatic ducts: autoradiographic and histochemical studies. J Anat 95:1–11
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Nagura H, Nakane PK, Brown WR (1979) Translocation of dimeric IgA through neoplastic colon cells in vitro. J Immunol 123:2359–2368
Nagura H, Tsutsumi Y, Shioda Y, Watanabe K (1983) Immunohistochemistry of gastric carcinomas and related diseases: novel distribution of carcinoembryonic antigen and secretory component of the surface of gastric cancer cells. J Histochem Cytochem 31:193–198
Nap M, TenHoor KA, Fleuren GJ (1983) Cross-reactivity with normal antigens in commercial anti-CEA sera, used for immunohistology. The need for tissue controls and absorptions. Am J Clin Pathol 79:25–31
O'Brien MJ, Zamcheck N, Bruke B, Kirkham SE, Saravis CA, Gorrtieb LS (1981) Immunocytochemical localization of carcinoembryonic antigen in benign and malignant colorectal tissues. Assessment of diagnostic value. Am J Clin Pathol 75:283–290
Poger ME, Hirsch BR, Lamm ME (1976) Synthesis of secretory component by colonic neoplasm. Am J Pathol 82:327–338
Rognum TO, Fausa O, Brandtzaeg P (1982) Immunohistochemical evaluation of carcinoembryonic antigen, secretory component, and epithelial IgA in tubular and villous large-bowel adenomas with different grade of dysplasia. Scan J Gastroenterol 17:341–348
Svenberg T (1976) Carcinoembryonic antigen-like substances of human bile. Isolation and partial characterization. Int J Cancer 17:588–596
Tourville DR, Adler RH, Bienenstock T, Tomasi TB Jr (1969) The human secretory immunoglobulin system: immunological location ofγA, secretory “piece” and lactoferrin in normal human tissues. J Exp Med 129:411–429
Tsutsumi Y, Nagura H, Osamura RY, Watanabe K, Yanaihara N (1982) Immunohistochemical observation of metaplastic lesions in the gallbladder. Digest Org Immunol 9:78–84 (in Japanese)
Tsutsumi Y, Nagura H, Watanabe K (1984) Immunohistochemical observation of carcinoembryonic antigen (CEA) and CEA-related substances in the pancreas. Digest Org Immunol 12:221–228 (in Japanese)
Wilson MB, Nakane PK (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In: Knapp W (ed) Immunofluorescence and related staining technique. Elsevier/North Holland Biochemical Press, Amsterdam, pp 215–225
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nagura, H., Tsutsumi, Y., Watanabe, K. et al. Immunohistochemistry of carcinoembryonic antigen, secretory component and lysozyme in benign and malignant common bile duct tissues. Vichows Archiv A Pathol Anat 403, 271–280 (1984). https://doi.org/10.1007/BF00694903
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00694903