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Luminal alkalinization in the intestine of the goby

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Summary

The rate of luminal alkalinization in vitro byGillichthys mirabilis posterior intestine as measured by a manual pH stat technique was 0.70±0.05 μEquiv/cm2 h; acidification of the mucosal medium was never observed. The rate of HCO −3 secretion (J HCO 3) was reduced by ouabain, serosally-applied DIDS, removal of serosal HCO −3 and replacement of media Cl− with gluconate. HCO −3 secretion was enhanced replacement of Cl− with isethionate and unaffected by mucosal DIDS, furosemide or acetazolamide.J HCO 3 was reduced at mucosal pH above or below 7.5. These results support active HCO −3 secretion via a Cl−/HCO −3 exchange mechanism on the basolateral membrane and a conductive exit pathway for HCO −3 , H+ or OH− on the apical membrane.

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Abbreviations

DIDS :

diisothiocyanostilbene-2,2′-disulfonic acid

TEP :

transepithelial potential

GBR :

Gillichthyts bicarbonate Ringer

GUR :

Gillichthys unbuffered, bicarbonate-free Ringer

GER :

Gillichthys EPPS-buffered, bicarbonate-free Ringer

EPPS :

N-(2-hydroxyethyl)piperazine-N′-3-propanesulfonic acid

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Authors and Affiliations

  1. Department of Biological Sciences, State University of New York at Buffalo, 109 Cooke Hall, 14260, Buffalo, New York, USA

    James M. Dixon & Christopher A. Loretz

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  1. James M. Dixon
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  2. Christopher A. Loretz
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Dixon, J.M., Loretz, C.A. Luminal alkalinization in the intestine of the goby. J Comp Physiol B 156, 803–811 (1986). https://doi.org/10.1007/BF00694254

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