Summary
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1.
Lipophorin was isolated from the hemolymph of diapausing larvae of the southwestern corn borer,Diatraea grandiosella, and shown to have a density of 1:11 g/ml and a molecular weight of 520,000.
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2.
The holoprotein was comprised of apolipoprotein-I (M r=231,000) and apolipoprotein-II (M r=74,000), and contained about 38% lipid (28% neutral lipids, 10% polar lipids), and 3% carbohydrate.
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3.
The major neutral lipids present were diacylglycerol and sterol, while the major polar lipids present were phosphatidyl choline, phosphatidyl ethanolamine, and sphingomyelin. Carotenoids were also present.
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4.
Apolipophorin-I and apolipophorin-II were immunochemically distinct. The holoprotein was immunochemically identical to a protein present in the larval hemolymph of two other species ofDiatraea.
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5.
A mean concentration of 5 mg lipophorin/ml hemolymph was determined using rocket immunoelectrophoresis.
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Dillwith, J.W., Lenz, C.J. & Chippendale, G.M. Isolation and characterization of lipophorin from the hemolymph of diapausing larvae of the southwestern corn borer,Diatraea grandiosella . J Comp Physiol B 156, 783–789 (1986). https://doi.org/10.1007/BF00694251
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DOI: https://doi.org/10.1007/BF00694251