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Ring gland and prothoracic gland sensitivity to interspecific prothoracicotropic hormone extracts

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Summary

Using the techniques of intraspecific in vitro activation of prothoracic glands and ring glands by serial dilutions of prothoracicotropic hormone (PTTH) extracts from pupalManduca sexta (Lepidoptera) and larvalSarcophaga bullata (Diptera), a dose-response of activation was observed for both species. In both species maximum activation was at 0.5 brain equivalents while the number of brain equivalents necessary for half maximal stimulation (ED50) was 0.20 forManduca and 0.15 forSarcophaga. When prothoracic glands or ring glands were challenged with interspecific PTTH extracts from a stage different from that of the gland donor, no dose-response of gland activation was observed. However, whenM. sexta larval prothoracic glands were challenged byS. bullata larval PTTH extract, activation was observed. The dose-response profile fell midway between the dose-response curves obtained for the intraspecific assays. Thus, PTTH extract from one insect has the ability to activate the prothoracic glands of an insect representing another order.

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Author notes

  1. Brian Roberts

    Present address: Department of Zoology, Monash University, 3168, Clayton, Victoria, Australia

Authors and Affiliations

  1. Department of Biology, The University of North Carolina at Chapel Hill, 27514, Chapel Hill, North Carolina, USA

    Brian Roberts & Lawrence I. Gilbert

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  1. Brian Roberts
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  2. Lawrence I. Gilbert
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Roberts, B., Gilbert, L.I. Ring gland and prothoracic gland sensitivity to interspecific prothoracicotropic hormone extracts. J Comp Physiol B 156, 767–771 (1986). https://doi.org/10.1007/BF00694249

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  • DOI: https://doi.org/10.1007/BF00694249

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