Journal of comparative physiology

, Volume 101, Issue 2, pp 147–160 | Cite as

On the mechanical properties ofLimulus solid cuticle

  • I. Joffe
  • H. R. Hepburn
  • S. O. Andersen


  1. 1.

    For mature animals where all fractions of the cuticle have a high insoluble protein content, the outer fraction is notably brittle while the middle and inner fractions exhibit extensive plastic flow on tensile deformation.

  2. 2.

    The composite tensile behaviour ofLimulus cuticle can be adequately described in terms of a simple law of mixtures where the three fractions are held together by means of a brittle glue at their interfaces.

  3. 3.

    No significant differences were observed between the alcohol-preserved material and specimens of fresh whole cuticle in terms of mechanical behaviour where both have equal amounts of insoluble protein.

  4. 4.

    The fracture behaviour of the whole cuticle is biologically analogous to case-hardened engineering materials.

  5. 5.

    None of the available histological criteria for distinguishing individual components of the cuticle were useful as indicators of either the extent of tanning of the mechanical properties ofLimulus cuticle.

  6. 6.

    All the protein in the cuticle ofLimulus becomes stabilized by cross-linking, and this process is important both in making the protein insoluble and in determining the mechanical properties of the cuticle.



Mechanical Property Brittle Protein Content Human Physiology Mechanical Behaviour 
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  1. Andersen, S. O.: Comparison between the sclerotization of adult and larval cuticle inSchistocerca gregaria. J. Insect Physiol.19, 1603–1614 (1973)Google Scholar
  2. Andersen, S. O.: Cuticular sclerotization in larval and adult locusts,Schistocerca gregaria. J. Insect Physiol.20, 1537–1552 (1974a)Google Scholar
  3. Andersen, S. O.: Evidence for two mechanisms of sclerotization in insect cuticle. Nature (Lond.)251, 507–508 (1974b)Google Scholar
  4. Andersen, S. O., Barrett, F. M.: The isolation of ketocatechols from insect cuticle and their possible role in sclerotization. J. Insect Physiol.17, 69–83 (1971)Google Scholar
  5. Cutler, B., Richards, A. G.: Sclerotization and the localization of brown and black colors in chelicerates (Arthropoda). Zool. Jb. Anat.89, 404–421 (1972)Google Scholar
  6. Hearmon, R. F. S., Barkas, W.: The effect of grain direction on the Young's moduli and rigidity moduli of beech and sitka spruce. Proc. phys. Soc.53, 674–680 (1941)Google Scholar
  7. Hepburn, H. R.: Some mechanical properties of crossed fibrillar chitin. J. Insect Physiol.18, 815–825 (1972)Google Scholar
  8. Hepburn, H. R., Ball, A.: On the structure and mechanical properties of beetle shells. J. Materials Sci.8, 618–623 (1973)Google Scholar
  9. Hepburn, H. R., Joffe, I.: Hardening of locust sclerites. J. Insect Physiol.20, 631–635 (1974a)Google Scholar
  10. Hepburn, H. R., Joffe, I.: Locust solid cuticle — a time sequence of mechanical properties. J. Insect Physiol.20, 497–506 (1974b)Google Scholar
  11. Joffe, I., Hepburn, H. R.: Observations on regenerated chitin films. J. Materials Sci.8, 1751–1754 (1973)Google Scholar
  12. Joffe, I., Hepburn, H. R., Nelson, K. J., Green, N.: Mechanical properties of a crustacean exoskeleton. Comp. Biochem. Physiol.50A, 545–549 (1975)Google Scholar
  13. Karlson, P., Sekeri, K. E., Richards, A. G., Richards, P. A.: The amino acid composition of the various types of cuticle ofLimulus polyphemus. J. Insect Physiol.15, 495–507 (1969)Google Scholar
  14. Larsen, N. J.: Isolation and characterization of proteins from the cuticle ofLimulus polyphemus L. Comp. Biochem. Physiol., in press (1975)Google Scholar
  15. Neville, A. C.: Cuticle ultrastructure in relation to the whole insect. Symp. R. ent. Soc. Lond.5, 17–39 (1970)Google Scholar
  16. Neville, A. C., Thomas, M. G., Zelazny, B.: Pore canal shape related to molecular architecture of arthropod cuticle. Tissue & Cell1, 183–200 (1969)Google Scholar
  17. Richards, A. G.: The integument of arthropods. Minneapolis: University of Minnesota Press 1951Google Scholar
  18. Richards, A. G.: Sclerotization and the localization of brown and black colors in insects. Zool. Jb. Anat.84, 25–62 (1967)Google Scholar
  19. Welinder, B. S.: Halogenated tyrosines from the cuticle ofLimulus polyphemus (L.). Biochem. biophys. Acta (Amst.)279, 491–497 (1972)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • I. Joffe
    • 1
  • H. R. Hepburn
    • 1
  • S. O. Andersen
    • 2
  1. 1.Department of PhysiologyUniversity of the WitwatersrandJohannesburgRepublic of South Africa
  2. 2.Zoophysiological Laboratory CUniversity of CopenhagenCopenhagenDenmark

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