Archives of Microbiology

, Volume 142, Issue 3, pp 295–301 | Cite as

Fermentation of acetylene by an obligate anaerobe,Pelobacter acetylenicus sp. nov.

  • Bernhard Schink
Original Papers


Four strains of strictly anaerobic Gram-negative rod-shaped non-sporeforming bacteria were enriched and isolated from marine and freshwater sediments with acetylene (ethine) as sole source of carbon and energy. Acetylene, acetoin, ethanolamine, choline, 1,2-propanediol, and glycerol were the only substrates utilized for growth, the latter two only in the presence of small amounts of acetate. Substrates were fermented by disproportionation to acetate and ethanol or the respective higher acids and alcohols. No cytochromes were detectable; the guanine plus cytosine content of the DNA was 57.1±0.2 mol%. Alcohol dehydrogenase, aldehyde dehydrogenase, phosphate acetyltransferase, and acetate kinase were found in high activities in cell-free extracts of acetylene-grown cells indicating that acetylene was metabolized via hydration to acetaldehyde. Ethanol was oxidized to acetate in syntrophic coculture with hydrogen-scavenging anaerobes. The new isolates are described as a new species in the genusPelobacter, P. acetylenicus.

Key words

Pelobacter acetylenicus species description Acetylene fermentation Anaerobic hydrocarbon degradation Acetylene hydratase Syntrophic ethanol oxidation 


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  1. American Public Health Association Inc (ed) (1969) Standard methods for the examination of water and wastewater including bottom sediments and sludge. New York, pp 604–609Google Scholar
  2. Atlas RM (1981) Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev 45:180–209Google Scholar
  3. Bayer O (1954) Sauerstoffverbindungen. II. Teil 1. Aldehyde. In: Müller E (ed) Methoden der organischen Chemie, Bd. 7, 1; (Houben-Weyl). Thieme, Stuttgart, pp 449–451Google Scholar
  4. Bergmeyer HU (1974) Methoden der enzymatischen Analyse, 3rd edn. Verlag Chemie, WeinheimGoogle Scholar
  5. Birch-Hirschfeld L (1932) Die Umsetzung von Acetylen durchMycobacterium lacticola. Zentbl Bakteriol Parasitenkd Infektionskr Hyg Abt 2, 86:113–130Google Scholar
  6. Cline JD (1969) Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 14:454–458Google Scholar
  7. Collins PA, Knowles CJ (1983) The utilization of nitriles and other aliphatic and aromatic nitriles and amides. J Gen Microbiol 129:711–718Google Scholar
  8. Culbertson CW, Zehnder AJB, Oremland RS (1981) Anaerobic oxidation of acetylene by estuarine sediments and enrichment cultures. Appl Environ Microbiol 41:396–403Google Scholar
  9. Davis JB, Yarbrough HF (1966) Anaerobic oxidation of hydrocarbons byDesulfovibrio desulfuricans. Chem Geol 1:137–144Google Scholar
  10. DeBont JAM, Peck MW (1980) Metabolism of acetylene byRhodococcus A 1. Arch Microbiol 127:99–104Google Scholar
  11. DeBont JAM, Primrose SB, Collins MD, Jones D (1980) Chemical studies on some bacteria which utilize lower unsaturated hydrocarbons. J Gen Microbiol 117:97–102Google Scholar
  12. Duncan CL, Strong DH (1968) Improved medium for sporulation ofClostridium perfringens. Appl Microbiol 16:82–89Google Scholar
  13. Eichler B, Schink B (1985) Fermentation of primary alcohols and diols and pure culture of syntrophically alcohol-oxidizing anaerobes. Arch Microbiol (submitted)Google Scholar
  14. Elleway RF, Sabine JR, Nicholas DJD (1971) Acetylene reduction by rumen microflora. Arch Mikrobiol 76:277–291Google Scholar
  15. Foster JW (1962) Hydrocarbons as substrates for microorganisms. Antonie van Leeuwenhoek 7 Microbiol Serol 28:241–274Google Scholar
  16. Gibson DT (1975) Microbial degradation of hydrocarbons. In: Goldberg ED (ed) The nature of seawater. Dahlem Konferenzen, Berlin. Abakon Verlagsgescllschaft. Berlin, pp 667–696Google Scholar
  17. Goa J (1953) A microbiuret method for protein determination; determination of total protein in cerebrospinal fluid. Scand J Clin Lab Invest 5:218–222Google Scholar
  18. Gordon AJ, Ford RA (1972) The chemist's companion. A handbook of practical data, techniques, and references. Wiley, New York LondonGoogle Scholar
  19. Hollaus F, Sleytr U (1972) On the taxonomy and fine structure of some hyperthermophilic saccharolytic clostridia. Arch Mikrobiol 86:129–146Google Scholar
  20. Kanner D, Bartha R (1979) Growth ofNocardia rhodochrous on acetylene gas. J Bacteriol 139:225–230Google Scholar
  21. Kanner D, Bartha R (1982) Metabolism of acetylene byNocardia rhodochrous. J Bacteriol 150:989–992Google Scholar
  22. Kuenen JG, Veldkamp H (1972)Thiomicrospira pelophila nov. gen., nov. sp., a new obligately chemolithotrophic colourless sulfur bacterium. Antonie van Leeuwenhoek 7 Microbiol Serol 38:241–256Google Scholar
  23. Miller JM, Gray DO (1982) The utilization of nitriles and amides by aRhodococcus species. J Gen Microbiol 128:1803–1809Google Scholar
  24. Novelli GD, ZoBell CE (1944) Assimilation of petroleum hydrocarbons by sulfate-reducing bacteria. J Bacteriol 47:447–448Google Scholar
  25. Oberlies G, Fuchs G, Thauer RK (1980) Acetate thiokinase and the assimilation of acetate inMethanobacterium thermoautotrophicum. Arch Microbiol 128:248–252Google Scholar
  26. Odom JM, Peck HD (1981) Localization of dehydrogenases, reductases and electron transfer components in the sulfate-reducing bacteriumDesulfovibrio gigas. J Bacteriol 147:161–169Google Scholar
  27. Oremland RS, Taylor BF (1975) Inhibition of methanogenesis in marine sediments by acetylene and ethylene: validity of the acetylene reduction assay for anaerobic microcosms. Appl Microbiol 30:707–709Google Scholar
  28. Perry JJ (1979) Microbial cooxidations involving hydrocarbons. Microbiol Rev 43:59–79Google Scholar
  29. Pfennig N (1978)Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 28:283–288Google Scholar
  30. Rosenfeld WD (1947) Anaerobic oxidation of hydrocarbons by sulfate reducing bacteria. J Bacteriol 54:664–665Google Scholar
  31. Schink B (1984) Fermentation of 2,3-butanediol byPelobacter carbinolicus sp. nov. andPelobacter propionicus, sp. nov., and evidence for propionate formation from C2 compounds. Arch Microbiol 137:33–41Google Scholar
  32. Schink B (1985a) Degradation of unsaturated hydrocarbons by methanogenic enrichment cultures. FEMS Microbiol Ecol 31 (in press)Google Scholar
  33. Schink B (1985b) Inhibition of methanogenesis by ethylene and other unsaturated hydrocarbons. FEMS Microbiol Ecol 31 (in press)Google Scholar
  34. Schink B, Pfennig N (1982) Fermentation of trihydroxybenzenes byPelobacter acidigallici gen. nov. sp. nov., a new strictly anaerobic, non-sporeforming bacterium. Arch Microbiol 133: 195–201Google Scholar
  35. Schink B, Schlegel HG (1979) The membrane-bound hydrogenase ofAlcaligenes eutrophus. I. Solubilization, purification, and biochemical properties. Biochim Biophys Acta 567:315–324Google Scholar
  36. Schink B, Stieb M (1983) Fermentative degradation of polyethylene glycol by a new, strictly anaerobic, Gram-negative, non-sporeforming bacterium,Pelobacter venetianus sp. nov. Appl Environ Microbiol 45:1905–1913Google Scholar
  37. Schütz H, Radler F (1984) Propanediol-1,2-dehydratase and metabolism of glycerol ofLactobacillus brevis. Arch Microbiol 139:366–370Google Scholar
  38. Sprott GD, Jarrell KF, Shaw KM, Knowles R (1982) Acetylene as an inhibitor of methanogenic bacteria. J Gen Microbiol 128:2453–2462Google Scholar
  39. Stouthamer AH (1979) The search for correlation between theoretical and experimental growth yields. In: Quayle JR (ed) International review of biochemistry, microbial biochemistry, vol 21. University Park Press, Baltimore, pp 1–47Google Scholar
  40. Thauer RK, Jungermann K, Decker K (1977) Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev 41:100–180Google Scholar
  41. Toraya T, Honda S, Fukui S (1979) Fermentation of 1,2-propanediol and 1,2-ethanediol by some genera of Enterobacteriacea, involving coenzyme B12-dependent diol dehydratase. J Bacteriol 139:39–47Google Scholar
  42. Watanabe I, De Guzman MR (1980) Effect of nitrate on acetylene disappearance from anaerobic soil. Soil Biol Biochem 12:193–194Google Scholar
  43. Widdel F, Pfennig N (1981) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environments. Description ofDesulfobacter postgatei gen. nov., sp. nov. Arch Microbiol 129:395–400Google Scholar
  44. Widdel F, Kohring G-W, Mayer F (1983) Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. III. Characterization of the filamentous glidingDesulfonema limicola gen. nov. sp. nov., andDesulfonema magnum sp. nov. Arch Microbiol 134:286–294Google Scholar
  45. Yamada EW, Jakoby WB (1958) Enzymatic utilization of acetylenic compounds. I. An enzyme converting acetylenedicarboxylic acid to pyruvate. J Biol Chem 233:706–711Google Scholar
  46. ZoBell CE (1946) Action of microorganisms on hydrocarbons. Bacteriol Rev 10:1–49Google Scholar
  47. ZoBell CE, Prokop JF (1966) Microbial oxidation of mineral oils in Barataria Bay bottom deposits. Z Allg Mikrobiol 6:143–162Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Bernhard Schink
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzFederal Republic of Germany

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