Molecular and General Genetics MGG

, Volume 152, Issue 3, pp 325–329 | Cite as

Chromosomal and extrachromosomal inheritance of erythromycin-resistance in the “Petite-Negative” yeastKluyveromyces lactis

  • L. Del Giudice
  • A. Brunner


Spontaneously arising erythromycin-resistant mutants were isolated in the “petite-negative” yeastKluyveromyces lactis. Two independently arising mutants were studied, in one erythromycin-resistance was conferred by a single dominant nuclear gene, and in the other the resistance was extrachromosomally inherited. In fermentable medium growth of sensitive and resistant strains in presence or absence of erythromycin does not qualitatively changes the cytochrome absorption spectra, whereas oxygen uptake of parental strains growing in glucose-medium is affected by the drug.

The importance of “petite-negative” yeasts likeKluyveromyces lactis for the study of nucleo-cytoplasmic interrelations is discussed.


Oxygen Absorption Spectrum Medium Growth Erythromycin Lactis 
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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • L. Del Giudice
    • 1
  • A. Brunner
    • 2
  1. 1.International Institute of Genetics and BiophysicsC.N.R.NaplesItaly
  2. 2.Dept. of Molecular SciencesUniversity of WarwickCoventryUK

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