Summary
In order to find new genetic loci and functions on the yeast mitochondrial DNA, especially mutations affecting the mitochondrial protein synthesis apparatus, temperature sensitive mutants have been isolated after MnCl2 mutagenesis and mitochondrial and nuclear mutants classified according to their pattern of recombination with three rho- tester strains.
Eighteen cold- and heat-sensitive respiratory deficient mitochondrial mutants have been isolated and localized on the mitochondrial genome by deletion mapping using 113 rho- strains. Eight of them appear to represent new loci, among which some are probably mutations of the tRNA and rRNA genes.
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Bolotin-Fukuhara, M., Faye, G., Fukuhara, H.: Localization of some mitochondrial mutations in relation to transfer and ribosomal RNA genes inSaccharomyces cerevisiae, in: The Genetic function of mitochondrial DNA, Eds. C. Saccone and A.M. Kroon, pp. 243–250. Amsterdam, the Netherlands: Elsevier (North-Holland Biomedical Press)
Faye, G., Bolotin-Fukuhara, M., Fukuhara, H.: Mitochondrial mutations that affect mitochondrial transfer ribonucleic acids inSaccharomyces cerevisiae, in: The genetics and the biogenesis of chloroplasts and mitochondria. Eds. T. Bücher, W. Neupert, W. Sebald and S. Werner. Amsterdam: North-Holland 1976
Faye, G., Kujawa, C., Fukuhara, H.: Physical and Genetic organization of petite and grande yeast mitochondrial DNA. IV.: in vivo transcription products of mitochondrial DNA and localization of 23S ribosomal RNA in petite mutants ofSaccharomyces cerevisiae. J. molec. Biol.88, 185–203 (1974)
Flury, U., Mahler, H.R., Feldman, F.: A novel resiratory-deficient mutant ofSaccharomyces cerevisiae. J. biol. Chem.249, 6130–6137 (1974)
Fukuhara, H., Bolotin-Fukuhara, M., Hsu, H.J., Rabinowitz, M. Deletion mapping of mitochondrial transfer RNA genes inSaccharomyces cerevisiae by means of cytoplasmic petite mutants. Molec. gen. Genet.145, 7–17 (1976)
Fukuhara, H., Faye, G., Michel, F., Lazowska, J., Deutsch, J., Bolotin-Fukuhara, M., Slonimski, P.P.: Physical and genetic organization of petite and grande yeast mitochondrial DNA. I. Studies by DNA-RNA hybridization. Molec. gen. Genet.130, 215–238 (1974)
Handwerker, A., Schweyen, R.J., Wolf, K., Kaudewitz, F.: Evidence for an extrakaryotic mutation affecting the maintenance of the rho factor in yeast. J. Bact.113, 1307–1310 (1973)
Jaskunas, S.R., Nomura, M., Davies, J.: Genetics of bacterial ribosomes, in: Ribosomes, eds. M. Nomura, A. Tissières, P. Lengyel, pp. 333–368. Cold Springer Harbor, N.Y.: Cold Spring Harbor Laboratory 1974
Kotylak, Z., Slonimski, P.P.: Joint control of cytochromes a and b by a unique mitochondrial DNA region comprising four genetic loci, in: The genetic function of mitochondrial DNA. Eds. C. Saccone and A.M. Kroon pp. 143–154. Amsterdam, the Netherlands: Elsevier (North-Holland Biochemical Press) 1976
Martin, N., Rabinowitz, M.: Transfer RNAs in yeast mitochondria, in: The genetics and biogenesis of chloroplasts and mitochondria. Ed. T. Tücher, W. Neupert, W. Sebald and S. Werner. Amsterdam: North-Holland 1976
McLaughlin, C.S.: Yeast ribosomes: Genetics, in: Ribosomes, Eds. M. Nomura, A. Tissières, P. Lengyel, pp. 815–828. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1974
Monnerot, M., Schweyen, R.J., Fukuhara, H.: Mapping of mutation tsm-8 with respect to transfer RNA genes on the mitochondrial DNA ofSaccharomyces cerevisiae. Molec. gen. Genet.152, 307–309 (1977)
Morimoto, R., Lewin, A., Hsu, H.J., Rabinowitz, M., Fukuhara, H.: Restriction endonuclease analysis of mitochondrial DNA from grande and genetically characterized cytoplasmic petite clones ofSaccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.)72, 3868–3872 (1975)
Putrament, A., Baranowska, H., Prazmo, W.: Induction by manganese of mitochondrial antibiotic resistant mutations in yeast. Molec. gen. Genet.126, 357–366 (1973)
Sanders, J.P.M., Heyting, C., DiFranco, A., Borst, P., Slonimski, P.P.: The organization of genes in yeast mitochondrial DNA, in: The genetic function of mitochondrial DNA. Eds., C. Saccone and A.M. Kroon. Amsterdam: Elsevier (North-Holland Biomedical Press) 1976
Slonimski, P.P., Tzagoloff, A.: Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and for coenzyme QH2-cytochrome c reductase. Europ. J. Biochem.61, 27–41 (1976)
Storm, E.M., Marmur, J.: A temperature sensitive mitochondrial mutation ofSaccharomyces cerevisiae. Biochem. biophys. Res. Commun64, 752–759 (1975)
Tzagoloff, A., Akai, A., Needleman, R.B.: Properties of cytoplasmic mutants ofSaccharomyces cerevisiae with specific lesions in cytochrome oxidase. Proc. nat. Acad. Sci. (Wash.)72, 2054–2057 (1975a)
Tzagoloff, A., Akai, A., Needleman, R.: Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants ofSaccharomyces cerevisiae with specific defects in mitochondrial functions. J. Bact.122, 826–831 (1975b)
Williamson, D.H., Maroudas, N.G., Wikie, D.: Induction of the cytoplasmic petite mutation inSaccharomyces cerevisiae by the antibacterial antibiotics Erythromycin and Chloramphenicol. Molec. gen. Genet.111, 209–223 (1971)
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Communicated by F. Kaudewitz
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Bolotin-Fukuhara, M., Faye, G. & Fukuhara, H. Temperature-sensitive respiratory-deficient mitochondrial mutations: Isolation and genetic mapping. Molec. Gen. Genet. 152, 295–305 (1977). https://doi.org/10.1007/BF00693083
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DOI: https://doi.org/10.1007/BF00693083