Molecular and General Genetics MGG

, Volume 152, Issue 3, pp 245–251 | Cite as

Complementation betweenPhycomyces mutants of mating type (+) with abnormal phototropism

  • T. Ootaki
  • T. Kinno
  • K. Yoshida
  • A. P. Eslava


Twelve mutants ofPhycomyces blakesleeanus with defects in sporangiophore phototropism (genotypemad) were obtained from a wild type of the (+) mating type by mutagenesis with nitrosoguanidine. These mutants were tested for genetic complementation against standard (+)mad mutants derived from sexual crosses between the isogenic (+) strain and established (-)mad mutants (Ootaki et al., 1974; Eslava et al., 1976). Heterokaryons for complementation tests were obtained by grafting stage I sporangiophores. The (+) mutants were also investigated for their sensory responses such as photoinduction of sporangiophores and avoidance. The mutants were grouped into two classes, based on the phenotypic classification scheme of Bergman et al. (1973). There were eleven class 1.2 mutants and one class 2 mutant. Complementation tests revealed that all eleven class 1.2 mutants carry the genemadC and the class 2 mutant carriesmadD. There was no evidence that any were double mutants. These results are consistent with the phenotypic classification and with the complementation results of themad mutants of the (-) mating type.


Classification Scheme Double Mutant Mating Type Sensory Response Complementation Test 
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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • T. Ootaki
    • 1
  • T. Kinno
    • 1
  • K. Yoshida
    • 1
  • A. P. Eslava
    • 2
  1. 1.Biology Laboratory, Education FacultyYamagata UniversityYamagataJapan
  2. 2.Departmento de Genética, Facultated de BiologiaUniversidad de OviedoLeonSpain

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