Summary
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1.
Influx of14C-labeled serine and leucine into axenic larvae ofStrongylocentrotus purpuratus occurs from substrate concentrations as low as 125 nmolar. The relation between substrate concentration and the rate of influx has the form of a rectangular hyperbola and is well described by the Michaelis-Menten equation. The apparent kinetic constantsJ i max andK t are both dependent on the sodium concentration of the medium.
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2.
Analysis of the relation between the measured influx of serine or leucine, and sodium concentration suggests that two sodium ions are transported per amino acid. The data also suggest cooperative interaction between the binding sites for both sodium and the amino acid substrate.
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3.
We provide the first clear evidence of sodium coupled net entry of 9 neutral amino acids, as determined by high performance liquid chromatography. We propose a model for transport of the form (Na+)2·(carrier)·(S) and provide evidence that this quaternary complex is translocated intact across the cell surface.
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Davis, J.P., Keenan, C.L. & Stephens, G.C. Na+-dependent amino acid transport in bacteria-free sea urchin larvae. J Comp Physiol B 156, 121–127 (1985). https://doi.org/10.1007/BF00692934
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DOI: https://doi.org/10.1007/BF00692934