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The conversion of wax scales into comb wax by African honeybees

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Summary

  1. 1.

    The conversion of virgin beeswax scales into finished comb wax has been analysed by crystallographic, mechanical and chemical means.

  2. 2.

    The effects of mandibulation by the honeybees are to transform the texturally anisotropic scale into planar isotropic comb.

  3. 3.

    Both waxes contain proteins. They differ in gross lipid composition.

  4. 4.

    The saliva added to the wax by the bees contains material with very probable lipolytic activity that reduces the diglyceride pool of the scale wax with a corresponding increase in the monoglyceride fraction of the comb wax.

  5. 5.

    The combined effects of the crystallographic and chemical changes on the mechanical properties of the wax are as follows. Scale wax is as strong at 23°C as comb wax but the latter has twice the stiffness and is less distensible than the former. The energy to fracture comb wax (an index of the work bees must invest to shape it) is only half that of scale wax over the range of temperatures likely to impinge upon the nests of honeybees.

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References

  • Brewster D (1815) Experiments on the depolarisation of light as exhibited by various mineral, animal and vegetable bodies, with a reference to the general principle of polarisation. Philos Trans R Soc 104:187–211

    Google Scholar 

  • Buttel-Reepen H (1915) Leben und Wesen der Bienen. Vieweg & Sohn, Braunschweig

    Google Scholar 

  • Casteel DB (1912) The manipulation of the wax scales of the honeybee. Circ US Bur Ent 161:1–13

    Google Scholar 

  • Donhoff E (1854) Etwas über den Hergang beim Zellenbau. Schweiz Bienenztg 15:170–171

    Google Scholar 

  • Dujardin F (1850) Sur l'étude microscopique de la cire appliquée à la recherche de cette substance chez les animaux et les vegetaux. CR Acad Sci Paris 30:172–173

    Google Scholar 

  • Folch J, Lees M, Sloan Stanley GM (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–501

    PubMed  CAS  Google Scholar 

  • Friedrich W (1913) Eine neue Interferenzerscheinung bei Röntgenstrahlen. Z Phys 14:317–319

    CAS  Google Scholar 

  • Gaubert P (1910) Sur les cristaux mous et sur la mesure de leurs indices de réfraction. CR Acad Sci Paris 151:532–534

    Google Scholar 

  • Hepburn HR, Chandler HD (1980) Material testing of arthropod cuticle preparations. In: Miller T (ed) Cuticle techniques in arthropods. Springer-Verlag, Berlin, pp 1–44

    Google Scholar 

  • Hepburn HR, Kurstjens SP (1984) On the strength of propolis (bee glue). Naturwissenschaften 71:591

    Article  Google Scholar 

  • Hepburn HR, Armstrong E, Kurstjens SP (1983) The ductility of native beeswax is opitimally related to honeybee colony temperature. S Afri J Sci 79:416–417

    Google Scholar 

  • Holz H (1878) Das Organ der Wachsbildung. Bienenztg 34:183–184

    Google Scholar 

  • Hornbostel HC (1744) Neue Entdeckung wie das Wachs von den Bienen kommt. Hamburger vermischte Bibliothek 2:45–62

    Google Scholar 

  • Huber F (1814) Nouvelles observations sur les abeilles. Dadant, Hamilton (Dadant translation)

    Google Scholar 

  • Hunter J (1792) Observations on bees. Philos Trans R Soc 82:128–196

    Google Scholar 

  • Joffe I, Hepburn HR (1974) A simple low-cost tensometer for biomaterial testing. Experientia 30:113–114

    Article  Google Scholar 

  • Jordan R (1962) Anomale Wachsplättehen. Bienenvater 63:299–301

    Google Scholar 

  • Kohlhaas R (1938) Röntgenographische Untersuchung von definierten Einkristallen des Palmitinsäure-Cetylesters. Z Krist 98:418–438

    CAS  Google Scholar 

  • Lineburg B (1924) Comb building. Am Bee J 64:271–272

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  • Philipp PW (1935) Neues vom Wachsschüppchen und der Wachszange. Rhein Bienen-Ztg 8:241–244

    Google Scholar 

  • Rösch GA (1927) Über die Bautätigkeit im Bienenvolk und das Alter der Baubienen. Weiterer Beitrag zur Frage nach der Arbeitsteilung im Bienenstaat. Z Vergl Physiol 6:265–298

    Article  Google Scholar 

  • Schmidt WJ (1924) Die Bausteine des Tierkörpers im polarisierten Lichte. Bonn (cited from Schmidt 1941)

  • Schmidt WJ (1941) Polarisationsoptische Versuche mit Bienenwachs. Kolloid Z 100:140–151

    Article  Google Scholar 

  • Schoening FRL (1980) The x-ray diffraction pattern and deformation texture of beeswax. S Afr J Sci 76:262–265

    CAS  Google Scholar 

  • Storry J, Tuckley B (1967) Thin-layer chromatography of plasma lipids by single development. Lipids 2:501–502

    CAS  Google Scholar 

  • Tulloch AP (1979) Beeswax-composition and analysis. Bee World 61:47–62

    Google Scholar 

  • Woog P, Yannaquis N (1935) Sur l'orientation des molécules de la cire d'abeille. CR Acad Sci Paris 201:1400–1402

    CAS  Google Scholar 

  • Woog P, Yannaquis N (1936a) Orientation des molécules de la cire d'abeille et répercussion sur la solidité des rayons. CR Acad Sci Paris 202:76–78

    Google Scholar 

  • Woog P, Yannaquis N (1936b) Orientations des molécules de la cire d'abeille. Répercussions sur la construction des rayons. Arch Phys Biol 13:134–149

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Physiology, University of the Witwatersrand, Johannesburg, South Africa

    S. P. Kurstjens & H. R. Hepburn

  2. Department of Medical Biochemistry, University of the Witwatersrand, Johannesburg, South Africa

    S. P. Kurstjens & B. C. Davidson

  3. Department of Physics, University of the Witwatersrand, Johannesburg, South Africa

    F. R. L. Schoening

Authors
  1. S. P. Kurstjens
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  2. H. R. Hepburn
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  3. F. R. L. Schoening
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  4. B. C. Davidson
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Kurstjens, S.P., Hepburn, H.R., Schoening, F.R.L. et al. The conversion of wax scales into comb wax by African honeybees. J Comp Physiol B 156, 95–102 (1985). https://doi.org/10.1007/BF00692930

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  • DOI: https://doi.org/10.1007/BF00692930

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