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A study of the effects of water carbonate alkalinity on some parameters of blood acid-base status in rainbow trout (Salmo gairdneri R.)

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Summary

The effects of two levels of water carbonate alkalinity (CA=0.3–0.5 meq·l−1 and CA=12–13.5 meq·l−1) on arterial blood acid-base status (\(Pa_{CO_2}\), pHa, [HCO −3 ]+[CO −−3 ]), oxygen consumption (\(\dot M_{O_2 } \)) and plasma ionic composition (Na+, K+, Cl−) were investigated in trout living in normoxic-normocapnic water at 15°C.

The results show that a high level of carbonate alkalinity induced a decrease in\(Pa_{CO_2}\) and a situation of mixed respiratory and metabolic acidosis compared to that in low CA water.

These changes are accompanied by significant changes in ionic composition but the levels of oxygen consumption are unchanged.

The role of the different capacitance coefficients of water for CO2 and the effects of the different ionic composition of water on ionic and gascous exchanges are discussed.

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Abbreviations

\(PE_{CO_2}\) :

\(PI_{CO_2}\) partial pressure of carbon dioxide in inspired and expried water

\(Pin_{O_2}\) :

\(Pout_{O_2}\) partial pressure of oxygen in water entering and leaving the respirometers

\(Pw_{O_2}\) :

\(Pw_{CO_2}\)

\(Cw_{CO_2}\) :

pHw partial pressure and concentration of oxygen and carbon dioxide and pH in water

\(Pa_{CO_2}\) :

pHa partial pressure of carbon dioxide and pH in arterial blood

\(\alpha w_{O_2}\) :

\(\alpha w_{CO_2}\) oxygen and carbon dioxide solubility coefficients in water

\(\alpha p_{CO_2}\) :

solubility coefficient of carbon dioxide in blood plasma

\(\beta w_{CO_2}\) :

capacitance coefficient of carbon dioxide in water

TA :

titration alkalinity in water

CA :

carbonate alkalinity in water

\(\dot M_{CO_2 } \) :

rate of CO2 output

\(\dot M_{O_2 } \) :

rate of O2 uptake

R :

respiratory exchange ratio

V W :

ventilatory flow rate

Q W :

water flow rate in the chamber

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Authors and Affiliations

  1. Laboratoire de Physiologie Animale, Faculté des Sciences et Techniques, F-29283, Brest Cédex, France

    Serge Thomas & Joseph Poupin

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  1. Serge Thomas
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  2. Joseph Poupin
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Equipe de Recherche associée au CNRS N° 070622

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Thomas, S., Poupin, J. A study of the effects of water carbonate alkalinity on some parameters of blood acid-base status in rainbow trout (Salmo gairdneri R.). J Comp Physiol B 156, 29–34 (1985). https://doi.org/10.1007/BF00692923

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