Acta Neuropathologica

, Volume 74, Issue 2, pp 194–196 | Cite as

Autoclaved, formol-fixed scrapie mouse brain is suitable for histopathological examination, but may still be infective

  • D. M. Taylor
  • P. A. McBride
Regular Papers


Autoclaving Creutzfeldt-Jakob disease (CJD)-infected brain after fixation has been proposed previously as a means of making it safe for handling in the laboratory, while preserving its microscopic integrity. However, the practice which was recommended (126°C for 30 min) would be unlikely to achieve complete decontamination. Autoclaving scrapie mouse brain (an analogue for CJD) to the higher, recommended standard of 134–138°C for 18 min was found to lead to some tissue damage but not sufficient to prevent meaningful qualitative and quantitative study of the histopathological lesions.

Key words

Scrapie Autoclaving Histopathology 


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • D. M. Taylor
    • 1
  • P. A. McBride
    • 1
  1. 1.AFRC and MRC Neuropathologenesis UnitEdinburghUK

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