Release of prostaglandins by bradykinin as an intrinsic mechanism of its algesic effect

  • F. Lembeck
  • H. Popper
  • H. Juan


  1. 1.

    The release of PGs from the isolated perfused rabbit ear was measured by means of a radioimmunoassay.

  2. 2.

    Bradykinin in dose dependent amounts released mainly PGE (presumably PGE1) and in much smaller amounts also PGF.

  3. 3.

    Bradykinin released similar amounts of PGE in innervated and chronically denervated ears.

  4. 4.

    Indomethacin completely prevented the PGE release by bradykinin.

  5. 5.

    ACh showed a much lower efficacy than bradykinin in releasing PGE and PGF. Synthetic substance P was devoid of any PGE releasing action.

  6. 6.

    It is concluded that bradykinin increases its own algesic action by a concomitant rapid stimulation of the PGE synthesis, thus providing a mechanism for the facilitation of its own algesic action.


Key words

Acetylcholine Afferent nerve endings Bradykinin Prostaglandin release 


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  1. Crunkhorn, P., Willis, A. L.: Cutaneous reactions to intradermal prostaglandins. Brit. J. Pharmacol.41, 49–56 (1971)Google Scholar
  2. Ferreira, S. H.: Prostaglandins, aspirin-like drugs and analgesia. Nature New Biol.240, 200–203 (1972)Google Scholar
  3. Ferreira, S. H., Moncada, S., Vane, J. R.: Indomethacin and aspirin abolish prostaglandin release from the spleen. Nature New Biol.231, 237–239 (1971)Google Scholar
  4. Ferreira, S. H., Moncada, S., Vane, J. R.: Prostaglandins and the mechanism of analgesia produced by aspirin-like drugs. Brit. J. Pharmacol.49, 86–97 (1973)Google Scholar
  5. Ferreira, S. H., Moncada, S., Vane, J. R.: Potentiation by prostaglandins of the nociceptive activity of bragykinin in the dog knee joint. Brit. J. Pharmacol.50, 461P (1971)Google Scholar
  6. Gryglewski, R. J., Korbut, R.: Prostaglandin feedback mechanism limits vasoconstrictor action of norepinephrine in perfused rabbit car. Experientia (Basel)31, 89–91 (1975)Google Scholar
  7. Jobke, A., Peskar, B. A., Peskar, B. M.: On the specifity of antisera against prostaglandins A2 and E2. FEBS Letters37, 192–196 (1973)Google Scholar
  8. Juan, H., Lembeck, F.: Effect of substances on paravascular pain receptors of the perfused rabbit ear. Naunyn-Schmiedeberg's Arch. Pharmacol.277, R 34 (1973)Google Scholar
  9. Juan, H., Lembeck, F.: Action of peptides and other algesic agents on paravascular pain receptors of the isolated perfused rabbit ear. Naunyn-Schmiedeberg's Arch. Pharmacol.283, 151–164 (1974)Google Scholar
  10. Juan, H., Lembeck, F.: Antagonism of PGF and PGE1 on paravascular pain receptors. Int. conference on prostaglandins, abstracts book, p. 16, Florence, may 26–30 1975Google Scholar
  11. Juan, H., Lembeck, F.: Release of prostaglandins from the isolated perfused rabbit ear by bradykinin and acetylcholine. Agents and Actions (in press, 1976a)Google Scholar
  12. Juan, H., Lembeck, F.: Polyphloretin phosphate reduces the algesic action of bradykinin by interfering with E-type prostaglandins. Agents and Actions (in press, 1976b)Google Scholar
  13. Lembeck, F.: Untersuchungen über die Auslösung afferenter Impulse. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.230, 1–9 (1957)Google Scholar
  14. Lembeck, F., Juan, H.: Interaction of prostaglandins and indomethacin with algesic substances. Naunyn-Schmiedeberg's Arch. Pharmacol.285, 301–313 (1974)Google Scholar
  15. McGiff, J. C., Terragno, N. A., Malik, K. U., Lonigro, A. J.: Release of prostaglandin E-like substance from canine kidney by bradykinin. Circulat. Res.31, 36–43 (1972)Google Scholar
  16. Moncada, S., Ferreira, S. H., Vane, J. R.: Sensitization of pain receptors of dog knee joint by prostaglandins. In: Prostaglandin synthetase inhibitors (H. J. Robinson and J. R. Vane, eds.), pp 189–195, New York: Raven Press 1974Google Scholar
  17. Moncada, S., Ferreira, S. H., Vane, J. R.: Inhibition of prostaglandin biosynthesis as the mechanism of analgesia of aspirinlike drugs in the dog knee joint. Europ. J. Pharmacol.31, 250–260 (1975)Google Scholar
  18. Peskar, B., Hertting, G.: Release of prostaglandins from isolated cat spleen by angiotensin and vasopressin. Naunyn-Schmiedeberg's Arch. Pharmacol.279, 227–234 (1973)Google Scholar
  19. Smith, J. B., Willis, A. L.: Aspirin selectively inhibits prostaglandin production in human platelets. Nature New Biol.231, 235–237 (1971)Google Scholar
  20. Vane, J. R.: Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nature New Biol.231, 232–235 (1971)Google Scholar
  21. Willis, A. L., Cornelsen, M.: Repeated injection of prostaglandin E2 in rat paws induces chronic swelling and a marked decrease in pain threshold. Prostaglandins,3, 353–357 (1973)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • F. Lembeck
    • 1
  • H. Popper
    • 1
  • H. Juan
    • 1
  1. 1.Institut für experimentelle und klinische Pharmakologie der Universität GrazGrazAustria

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