Journal of Comparative Physiology B

, Volume 156, Issue 5, pp 635–640 | Cite as

Glycolytic enzyme binding and metabolic control in anaerobiosis

  • William C. Plaxton
  • Kenneth B. Storey


Metabolic rate depression is a key survival strategy used by facultative anaerobes for enduring periods of environmental anoxia. In determining the molecular mechanisms of this phenomenon the role of enzyme binding to the subcellular particulate fraction was assessed in muscle tissues (ventricle and foot) of the anoxia tolerant marine gastropod,Busycotypus canaliculatum. Using two different methodologies for preparation, soluble versus particulate fractions of muscle were separated and assayed for their contents of eight glycolytic enzymes. Preparations from anoxic animals showed decreased percentages of enzymes associated with the particulate fraction as compared to controls; this was particularly pronounced for hexokinase and aldolase. A return to aerated seawater reversed this effect, and increased enzyme binding to the particulate fraction. The absence of a Pasteur effect in animal facultative anaerobes may be due, in part, to an anoxia-induced dissociation of enzymes from the particulate fraction of the cell promoting a decrease in glycolytic rate.


Human Physiology Metabolic Rate Muscle Tissue Survival Strategy Metabolic Control 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.







glycerol-3-phosphate dehydrogenase


pyruvate kinase


lactate dehydrogenase


alanopine dehydrogenase


octopine dehydrogenase




ethylenediamine tetraacetic acid


ethyleneglycol-bis-(2-amino ethylether)-N,N′-tetraacetic acid


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • William C. Plaxton
    • 1
  • Kenneth B. Storey
    • 1
  1. 1.Institute of Biochemistry and Department of BiologyCarleton UniversityOttawaCanada

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