Archives of Microbiology

, Volume 139, Issue 1, pp 44–47 | Cite as

Co-induction of sucrose transport and invertase activities in a thermophilic fungus,Thermomyces lanuginosus

  • P. Palanivelu
  • P. V. Balasubramanyam
  • Ramesh Maheshwari
Original Papers


The incorporation of sucrose into the thermophilic fungus,Thermomyces lanuginosus, occurred only in mycelia previously exposed to sucrose or raffinose. Sucrose uptake and invertase were inducible. Both activities appeared in sucrose-induced mycelia at about the same time. Both activities declined almost simultaneously following the exhaustion of sucrose in the medium. The sucrose-induced uptake system was specific for β-fructofuranosides as revealed by competition with various sugars. The induction of sucrose uptake system was blocked by cycloheximide, showing that it was dependent on new protein synthesis. Transport of sucrose did not seem to be dependent on ATP. Rather, uptake of this sugar seemed to be driven by a proton gradient across the plasma membrane. The uptake system showed Michaelis-Menten kinetics.

Key words

Sucrose uptake Transport Invertase Thermomyces lanuginosus Thermophilic fungus 



carbonylcyanide p-trifluoromethylphenyl hydrazone




Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Cooney DG, Emerson R (1964) Thermophilic fungi. Freeman and Company, San FranciscoGoogle Scholar
  2. Höfer M, Misra PC (1978) Evidence for a proton/sugar symport in the yeastRhodotorula gracilis (glutinis). Biochem J 172:15–22Google Scholar
  3. Kotyk A, Michaljanicova D (1979) Uptake of trehalose bySaccharomyces cerevisiae. J Gen Microbiol 110:323–332Google Scholar
  4. Maheshwari R, Balasubramanyam PV, Palanivelu P (1983) Distinctive behaviour of invertase in a thermophilic fungus,Thermomyces lanuginosus. Arch Microbiol 134:255–260Google Scholar
  5. Roseman S (1972) Carbohydrate transport in bacterial cells. In: Greenberg DM (ed) Metabolic pathways, vol 6. Academic, New YorkGoogle Scholar
  6. Santos E, Rodriguez L, Elorza MV, Sentandreu R (1982) Uptake of sucrose bySaccharomyces cerevisiae Arch Biochem Biophys 216:652–660Google Scholar
  7. Scarborough GA (1980) Proton translocation catalyzed by the electrogenic ATPase in the plasma membrane ofNeurospora. Biochemistry 19:2925–2931Google Scholar
  8. Serrano R (1977) Energy requirements for maltose transport in yeast. Eur J Biochem 80:97–102Google Scholar
  9. Slayman CL, Slayman CW (1974) Depolarization of the plasma membrane ofNeurospora during active transport of glucose: evidence for a proton-dependent cotransport system. Proc Natl Acad Sci, USA 71:1935–1939Google Scholar
  10. Trevelyan WE, Procter DP, Harrison JS (1950) Detection of sugars on paper chromatograms. Nature (Lond) 166:444–445Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • P. Palanivelu
    • 1
  • P. V. Balasubramanyam
    • 1
  • Ramesh Maheshwari
    • 1
  1. 1.Department of BiochemistryIndian Institute of ScienceBangaloreIndia

Personalised recommendations