Stromal cell changes in human colorectal adenomas and carcinomas
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Structural changes in stromal cells during the development of human colorectal carcinomas were studied by light and electron microscopy. The results were as follows:
1. Stromal cells of the lamina propria in control subjects consisted principally of resting fibroblasts.
2. Stromal fibroblasts were mildly activated in adenomas with mild- moderate atypia, and more markedly in adenomas with severe atypia (carcinoma in situ).
3. In invasive adenocarcinomas, (a) desmoplastic reaction was induced, (b) stromal fibroblasts proliferated significantly and were activated showing enlarged nuclei and abundant rough endoplasmic reticulum, and (c) some smooth muscle cells were endowed with well-developed rough endoplasmic reticulum in their axial cytoplasm, resulting in a similar appearance to “myofibroblasts”.
4. Stromal fibroblasts in ulcerative colitis and proctitis were also activated.
Morphometric analysis revealed that activated fibroblasts significantly increased the areas of their nuclei and cytoplasm, and the perimeter of rough endoplasmic reticulum. These activated fibroblasts suggested a higher prediction of collagen and other connective tissue proteins. Bundles of microfilaments of actin type were readily found in fibroblasts in all cases examined. These filaments were most remarkable in the fibroblasts in the desmoplastic stroma of invasive adenocarcinoma and were considered to be one of the basic components of these cells. Relationships between fibroblasts, “myofibroblasts”, and smooth muscle cells are discussed.
Key wordsColorectal epithelial tumors Stroma Fibroblast Myofibroblast Smooth muscle cell
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- Böcker W, Strecker H (1975) Electron microscopy of uterine leiomyosarcomas. Virchows Arch [Pathol Anat] 367:59–71Google Scholar
- Gabbiani G, Hirschel BJ, Ryan GB, Statkov PR, Majno G (1972) Granulation tissue as a contractile organ. A study of structure and function. J Exp Med 135: 719–734Google Scholar
- Haudenschild CC, Schwartz SM (1979) Endothelial regeneration II. Restitution of endothelial continuity. Lab Invest 41:407–418Google Scholar
- Kaye GI, Lane N, Pascal RR (1968) Colonic pericryptal fibroblast sheath: replication, migration, and cytodifferentiation of a mesenchymal cell system in adult tissue II. Fine structural aspects of normal rabbit and human colon. Gastroenterology 54:852–865Google Scholar
- Lipper S, Kahn LB, Reddick RL (1980) The myofibroblast. Pathol Annu 15:409–441Google Scholar
- Majno G, Gabbiani G, Hirschel BJ, Ryan GB, Statkov PR (1971) Contraction of granulation tissue in vitro: similarity to smooth muscle. Science 173:548–550Google Scholar
- Meyrick B, Reid L (1980) Hypoxia-induced structural changes in the media and adventitia of the rat hilar pulmonary artery and their regression. Am J Pathol 100:151–178Google Scholar
- Morson BC (1976) Histological typing of intestinal tumors. International histological classification of tumors. No. 15 World Health Organization, GenevaGoogle Scholar
- Naito Y, Kino I (1982) Promoting effects of gastric cancer cells on collagen synthesis of fibroblasts in vitro (in Japanese). Tissue Cult Res Commun 1:16–17Google Scholar
- Ohtani H, Sasano N (1979) Myofibroblasts in human breast tumors. An ultrastructural study. Tohoku J Exp Med 128:123–137Google Scholar
- Ohtani H, Sasano N (1980) Myofibroblasts and myoepithelial cells in human breast carcinomas. An ultrastructural study. Virchows Arch [Pathol Anat] 385:247–261Google Scholar
- Ross R. Klebanoff SJ (1971) The smooth muscle cell. I. In vivo synthesis of connective tissue proteins. J Cell Biol 50:159–171Google Scholar
- Ross R (1971) The smooth muscle cell. II. Growth of smooth muscle in culture and formation of elastic fibers. J Cell Biol 50:172–186Google Scholar
- Ryan GB, Cliff WJ, Gabbiani G, Irlé C, Montandon D, Statkov PR, Majno G (1974) Myofibroblasts in human granulation tissue. Human Pathol 5:55–67Google Scholar
- Schürch W, Seemayer TA, Lagacé R (1981) Stromal myofibroblasts in primary invasive and metastatic cacinoma. A combined immunological, light and electron microscopic study. Virchows Arch [Pathol Anat] 391:125–139Google Scholar
- Seemayer TA, Lagacé R, Schürch W, Tremblay G (1979) Myofibroblasts in the stroma of invasive and metastatic carcinoma. A possible host response to neoplasia. Am J Surg Pathol 3:525–533Google Scholar
- Seemayer TA, Lagacé R, Schürch W, Thelmo WL (1980) The myofibroblast: biologic, pathologic, and theoretical considerations. Pathol Annu 15:443–470Google Scholar