Acta Neuropathologica

, Volume 12, Issue 2, pp 141–157 | Cite as

The behavior of acoustic neuroma in tissue culture

  • Humberto Cravioto
  • Ruby Lockwood
Original Investigations

Summary

A phase contrast and time-lapse cinematographic study of 50 acoustic nerve tumors cultured in Rose chambers and roller tubes, was made. In all cultures four main cell types were observed: 1. ameboid microglia-like cells; 2. slender spindle-shaped cells; 3. racketshaped cells, and 4. large kite-shaped cells. The spindle-shaped cells exhibited a rhythmic pulsatile activity and grouped themselves in a palisading fashion. The ameboid cells showed as fast writhing movement of their elongated, irregularly shaped cell body and a fast undulating movement of their membranous veil-like cytoplasmic extensions. These two cells were thought to be Schwann cells. The racket-shaped cells slowly but constantly changed shape. The origin of this cell was not determined. The kite-shaped cells were though to be fibroblasts. Although every culture showed all four cell types, one of them usually predominated over the other; in general however the fibroblastic cells tended to predominate in long-term cultures. No transformation of one cell type into the other was observed. A comparison between the types of cells grown in vitro from acoustic tumors and from normal peripheral nerve was made.

Key-Words

Tissue Culture Acoustic Neuroma Schwann Cell Tumor in vitro 

Zusammenfassung

Eine Phasenkontrast- und Zeitraffer-kinematographische Untersuchung von 50 in Rose-Kammern und Rollröhrchen gezüchteten Acusticustumoren wurde durchgeführt. In allen Kulturen fanden sich vier Hauptzelltypen: 1. amöboide Mikrogliazellen, 2. schlanke spindelförmige Zellen, 3. Racket-förmige Zellen und 4. große drachenförmige Zellen. Die spindeligen Zellen zeigten eine rhythmische pulsierende Aktivität und gruppierten sich in Palisadenform. Die amöboiden Zellen zeigten schnelle Bewegungen ihres langgestreckten, unregelmäßig geformten Zellkörpers und rasche undulierende Bewegung ihrer membranösen schleierartigen Cytoplasmaausläufer. Diese beiden Zellen werden als Schwann-Zellen interpretiert. Die Racket-förmigen Zellen zeigen langsam aber ständig wechselnde Form. Die Herkunft dieser Zelle ist unbekannt. Die drachenförmigen Zellen werden als Fibroblasten aufgefaßt. Fast jede Kultur zeigte alle vier Zelltypen, von denen gewöhnlich eine überwog. Im allgemeinen pflegten Fibroblasten in Langzeit-Kulturen zu überwiegen. Kein Übergang eines Zelltyps in einen anderen wurde beobachtet. Ein Vergleich zwischen den Zellformen des in vitro Wachstums von Acusticustumoren und normalen peripheren Nerven wurde durchgeführt.

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Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • Humberto Cravioto
    • 1
    • 2
  • Ruby Lockwood
    • 1
    • 2
  1. 1.Laboratory of Neuropathology, Department of Pathology, Neurology and NeurosurgeryUniversity of Southern California School of MedicineLos Angeles
  2. 2.New York University Medical CenterNew YorkU.S.A.

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