Summary
The high affinity of granulocytes of guinea pig and man to glass surfaces is modified by serum. Native serum contains both an adherence-promoting activity, which is related to complement, and components which reduce the adhesiveness of granulocytes. These components are stable at 56°C for 30 min and are tightly bound to the glass surface. β-Lipoproteins are candidates for this adherence reducing ability of serum. Adherence promotion by native serum is mediated by coating the glass surface with C3b/C3bi. Human granulocytes from the peripheral blood adhered to glass surfaces coated by native human or guinea pig serum with C3b/C3bi to almost the same extent as in the presence of native serum, but on guinea pig granulocytes elicited in the peritoneal cavity, a cell surface metalloproteinase degraded the C3b/C3bi, thus reducing the adhesiveness of these cells. This proteinase was inhibited by MgEDTA, DTT, and 1,10-phenanthroline, whereby the high adhesiveness of granulocytes was restored to C3b/C3bi-coated glass.
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Abbreviations
- BA :
-
benzamidine hydrochloride
- BTS :
-
Bacillus thuringiensis subtoxicus
- DTT :
-
dithiothreitol
- EAC :
-
ɛ-amino-caproic acid
- gp :
-
guinea pig
- LDL :
-
low density lipoproteins
- SEM :
-
scanning electron microscopy
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Ehlers, D., Sakowski, I. & Mohrig, W. Comparative studies on the adhesiveness of granulocytes of guinea pig and man. J Comp Physiol B 159, 481–489 (1989). https://doi.org/10.1007/BF00692420
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DOI: https://doi.org/10.1007/BF00692420