Comparative studies on the adhesiveness of granulocytes of guinea pig and man

Summary

The high affinity of granulocytes of guinea pig and man to glass surfaces is modified by serum. Native serum contains both an adherence-promoting activity, which is related to complement, and components which reduce the adhesiveness of granulocytes. These components are stable at 56°C for 30 min and are tightly bound to the glass surface. β-Lipoproteins are candidates for this adherence reducing ability of serum. Adherence promotion by native serum is mediated by coating the glass surface with C3b/C3bi. Human granulocytes from the peripheral blood adhered to glass surfaces coated by native human or guinea pig serum with C3b/C3bi to almost the same extent as in the presence of native serum, but on guinea pig granulocytes elicited in the peritoneal cavity, a cell surface metalloproteinase degraded the C3b/C3bi, thus reducing the adhesiveness of these cells. This proteinase was inhibited by MgEDTA, DTT, and 1,10-phenanthroline, whereby the high adhesiveness of granulocytes was restored to C3b/C3bi-coated glass.

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Abbreviations

BA :

benzamidine hydrochloride

BTS :

Bacillus thuringiensis subtoxicus

DTT :

dithiothreitol

EAC :

ɛ-amino-caproic acid

gp :

guinea pig

LDL :

low density lipoproteins

SEM :

scanning electron microscopy

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Ehlers, D., Sakowski, I. & Mohrig, W. Comparative studies on the adhesiveness of granulocytes of guinea pig and man. J Comp Physiol B 159, 481–489 (1989). https://doi.org/10.1007/BF00692420

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Key words

  • Adherence
  • Complement
  • Serum constituents
  • Metalloproteinases