Journal of Comparative Physiology B

, Volume 159, Issue 4, pp 481–489 | Cite as

Comparative studies on the adhesiveness of granulocytes of guinea pig and man

  • Dieter Ehlers
  • Ina Sakowski
  • Werher Mohrig
Article

Summary

The high affinity of granulocytes of guinea pig and man to glass surfaces is modified by serum. Native serum contains both an adherence-promoting activity, which is related to complement, and components which reduce the adhesiveness of granulocytes. These components are stable at 56°C for 30 min and are tightly bound to the glass surface. β-Lipoproteins are candidates for this adherence reducing ability of serum. Adherence promotion by native serum is mediated by coating the glass surface with C3b/C3bi. Human granulocytes from the peripheral blood adhered to glass surfaces coated by native human or guinea pig serum with C3b/C3bi to almost the same extent as in the presence of native serum, but on guinea pig granulocytes elicited in the peritoneal cavity, a cell surface metalloproteinase degraded the C3b/C3bi, thus reducing the adhesiveness of these cells. This proteinase was inhibited by MgEDTA, DTT, and 1,10-phenanthroline, whereby the high adhesiveness of granulocytes was restored to C3b/C3bi-coated glass.

Key words

Adherence Complement Serum constituents Metalloproteinases 

Abbreviations

BA

benzamidine hydrochloride

BTS

Bacillus thuringiensis subtoxicus

DTT

dithiothreitol

EAC

ɛ-amino-caproic acid

gp

guinea pig

LDL

low density lipoproteins

SEM

scanning electron microscopy

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Dieter Ehlers
    • 1
  • Ina Sakowski
    • 1
  • Werher Mohrig
    • 1
  1. 1.Sektion Biologie, Institut für ZoologieErnst-Moritz-Arndt-UniversitätGreifswaldGerman Democratic Republic

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