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Acta Neuropathologica

, Volume 25, Issue 4, pp 249–258 | Cite as

Biometrical and histochemical comparison between extra- and intra-fusal muscle fibres in denervated and re-innervated rat muscle

  • J. de Reuck
  • H. van der Eecken
  • H. Roels
Original Investigations

Summary

Biometrical and histochemical changes in extra- and intrafusal muscle fibres were determined in twenty adult rats during denervation and re-innervation. The right sciatic nerve was severed and at regular intervals the denervated gastrocnemius, soleus and peroneal muscles were examined and compared with the corresponding muscles of the non denervated limb.

Although the extra- and intra-fusal muscle fibres show the same histochemical changes, the atrophy of the intra-fusal muscle fibres is insignificant compared with the atrophy of the extra-fusal muscle fibres in the denervation phase. This could be explained by the small size of the intra-fusal fibres and the increased fluid resorption of the spindle.

In the re-innervation phase the same histochemical changes occur in both types of muscle fibres.

Key words

Denervation Atrophy Muscle Spindle Muscle Fibre Size Alkaline Phosphatase A.T.P.ase Cholinesterase Phosphorylase Succinate Dehydrogenase 

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References

  1. Adams, R. D., Denny-Brown, D., Pearson, C. M.: Diseases of muscle. A study of pathology, 2nd Ed. New York: Hoeber 1962Google Scholar
  2. Barka, T., Anderson, P. J.: Histochemistry, pp. 232. New York: Hoeber 1965Google Scholar
  3. Brzin, M., Tennyson, V., Duffy, D.: Acetylcholinesterase in frog sympathetic and dorsal rootganglia. J. Cell Biol.31, 215–242 (1966)Google Scholar
  4. Campa, J. F., Engel, K. W.: Histochemistry of motorneurons and interneurons in the cat lumbar spinal cord. Neurology (Minneap.)20, 559–568 (1970)Google Scholar
  5. Cazzato, G., Walton, J. N.: The pathology of the muscle spindle: a study of biopsy material in various muscular and neuromuscular diseases. J. neurol. Sci.7, 15–70 (1968)Google Scholar
  6. Cooper, S., Daniel, P. M.: Muscle spindles in man: their morphology in the lumbricals and the deep muscles of the neck. Brain86, 563–594 (1963)Google Scholar
  7. Dubowitz, V.: Histochemical aspects of muscle disease. In: Disorders of voluntary muscle, pp. 239–276. Ed. J. Walton. London: Churchill 1969Google Scholar
  8. Eadie, M. J., Penny, J. E., Kukums, J. R., Tyrer, J. H.: Measurement of oxidative enzyme activity in anterior horn cells. Excerpta Medica No237, II International Congress on Muscle Disease, vol.12, pp. 4–5 (1971)Google Scholar
  9. Greenfield, J. G., Shy, G. M., Alvord, E. C., Bergh, L.: An atlas of muscle pathology in neuromuscular diseases. Edingburgh: Livingstone 1957Google Scholar
  10. Green, E. C.: The anatomy of the rat. New York: Hafner 1968Google Scholar
  11. Gruner, E.: La structure fine du fuseau neuromusculaire humain. Rev. neurol.104, 490–507 (1961)Google Scholar
  12. Guth, L., Zalewski, A. A., Brown, W. C.: Quantitative changes in cholinesterase activity of denervated sole plate following implantation of nerve into muscle. Exp. Neurol.16, 136–147 (1966)Google Scholar
  13. Guth, L., Watson, Ph.: The influence of innervation of the soluble proteins of slow and fast muscle of the rat. Exp. Neurol.17, 107–117 (1967)Google Scholar
  14. Kennedy, W. R.: Innervation of normal human muscle spindles. Neurology (Minneap.)20, 463–475 (1970)Google Scholar
  15. Kennedy, W. R.: Innervation of muscle spindles in amyotrophic lateral sclerosis. Proc. Mayo Clin.46, 245–257 (1971)Google Scholar
  16. Lapresle, J., Milhaud, M.: Pathologie du fuseau neuro-musculaire. Rev. neurol.110, 97–122 (1964)Google Scholar
  17. Maier, A., Eldred, E., Edgerton, V. R.: The effects on muscle spindles of muscle atrophy and hypertrophy. Exp. Neurol.37, 100–123 (1972)Google Scholar
  18. Mair, W. G. P., Tomé, F. M. S.: Atlas of the ultrastructure of diseased human muscle. Edinburgh: Churchill Ed. 1972Google Scholar
  19. Margreth, A., Salvati, G.: Biochemical changes in slow muscle by re-innervation with fast nerve fibres. Excerpta Medica No 237, II International Congress on Muscle disease, vol. 64, p. 25 (1971)Google Scholar
  20. Moore, M. J., Rebeiz, J. J., Holden, M., Adams, R. D.: Biometric analyses of normal skeletal muscle. Acta neuropath. (Berl.)19, 51–69 (1971)Google Scholar
  21. Nachmias, V. T., Padykula, H. A.: A histochemical study of normal and denervated red and white muscles of the rat. J. biophys. biochem. Cytol.4, 47 (1958)Google Scholar
  22. Patel, A. N., Lalitha, V. S., Dastur, D. K.: The spindle in normal and pathological muscle: an assessment of the histological changes. Brain91, 737–750 (1968)Google Scholar
  23. Pearse, E.: Histochemistry: theoretical and applied, p. 910, London: Churchill 1960Google Scholar
  24. Rebeiz, J. J., Moore, M. J., Holden, E. M., Adams, R. D.: Variations in muscle status with age and systemic disease. Acta neuropath. (Berl.)22, 127–144 (1972)Google Scholar
  25. Romanul, F. C., Van der Meulen, J. P.: Slow and fast muscles after cross Innervation: Enzymatic and physiological changes. Arch. Neurol. (Chic.)17, 387–402 (1967)Google Scholar
  26. Saghal, V., Nayyar, R., Betts, H. B.: Histochemical profile of human intrafusal fibres. Excerpta Medica No 237: II International Congress on Muscle Disease, vol. 5, pp. 2–3 (1971)Google Scholar
  27. Siegel, S.: Non parametric statistics for the behavioral sciences “the Kolmogorov-Smirnov two sample test”, pp. 127–136. New York: McGraw-Hill 1956Google Scholar
  28. Stein, J. M., Padykula, H. A.: Histochemical classification of individual skeletal muscle fibres in the rat. Amer. J. Anat.110, 103 (1962)Google Scholar
  29. Takeuchi, T., Kuriaki, H.: Histochemical detection of phosphorylase in animal tissues. J. Histochem. Cytochem.3, 153 (1955)Google Scholar
  30. Tower, S. S.: Atrophy and degeneration in skeletal muscle. Amer. J. Anat.56, 1–43 (1935)Google Scholar
  31. Tower, S. S.: Atrophy and degeneration in the muscle spindle. Brain55, 77–90 (1932)Google Scholar
  32. Wachstein, M., Meisel, E.: Histochemistry of hepatic phosphatase at a physiological Ph. Amer. J. clin. Path.27, 13 (1957)Google Scholar
  33. Wohlfart, S.: Muscular atrophy in diseases of the lower motor neuron. Arch. Neurol. Psychiat. (Chic.)61, 599 (1949)Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • J. de Reuck
    • 1
    • 2
  • H. van der Eecken
    • 1
    • 2
  • H. Roels
    • 1
    • 2
  1. 1.Department of Neurology and Department of PathologyUniversity of GhentBelgium
  2. 2.Department of NeurologyAcademisch ZiekenhuisGent(Belgium)

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