Journal of Comparative Physiology B

, Volume 159, Issue 2, pp 237–242 | Cite as

Theamylase gene-enzyme system of fishes

II. Comparison of amylases from the mosquitofish, rat andDrosophila
  • Darrell G. Yardley
Article

Summary

Amylases from the mosquitofish (Gambusia affinis holbrooki, Pisces: Poeciliidae) and rat were purified and compared withDrosophila amylases in terms of structure and function. At the structural level, amino acid compositions of the three amylases were compared. At the functional level, amylase activities were compared on various substrates and in the presence of inhibitors.

While the amylases from all three organisms had properties typical of alpha-amylases, both structural and functional differences were observed. Using resemblance coefficients of distance and similarity from numerical taxonomy, it was determined that the amylases from the rat andDrosophila were more similar to each other than either was to amylase from the mosquitofish, and that structural differences between the amylases did not reflect functional differences, i.e. there was no correlation between amylase structural and functional distances.

Key words

Amylase Mosquitofish Rat Drosophila Structure Function 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Darrell G. Yardley
    • 1
  1. 1.Department of Biological SciencesClemson UniversityClemsonUSA

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