Acta Neuropathologica

, Volume 39, Issue 3, pp 281–287 | Cite as

Brain-associated cell surface antigens on neuroepithelial cells in a transplantable mouse teratoma

  • S. R. Vanden Berg
  • J. E. Hickey
  • M. M. Herman
Original Investigations

Summary

Mouse neonatal brain cell fractions enriched for surface membranes were used as immunogens to produce a heterologous immune serum. Following absorption to remove non-neural anti-mouse activity, this serum demonstrated by microcomplement fixation an anti-brain activity that was completely removed by absorption with neonatal mouse brain or with solid tumors of the mouse transplantable teratoma OTT-6050. Indirect immunofluorescence applied to living monolayer cultures of differentiating teratoma embryoid bodies showed the absorbed serum's reaction with neural cell surfaces only. In material studied with frozen sections, the absorbed serum recognized antigenic sites in all examined areas of both neonatal and adult mouse brain, and only within neuroepithelial cell populations of solid transplants of the teratoma.

Key words

Neuroepithelial differentiation Microcomplement fixation Indirect immunofluorescence 

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References

  1. 1.
    Akeson, R., Herschman, H. R.: Modulation of cell-surface antigens of a murine neuroblastoma. Proc. nat. Acad. Sci. (Wash.)71, 187–191 (1974)Google Scholar
  2. 2.
    Artzt, K., Dubois, P., Bennett, D., Condamine, H., Babinet, C., Jacob, F.: Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture. Proc. nat. Acad. Sci. (Wash.)70, 2988–2992 (1973)Google Scholar
  3. 3.
    Babinet, C., Condamine, H., Fellous, M., Gachelin, G., Kemler, R., Jacob, F.: Expression of a cell surface antigen common to primitive mouse teratocarcinoma cells and cleavage embryos during embryogenesis and spermatogenesis: In: Teratomas and differentiation (eds. M. I. Sherman and D. Solter), pp. 101–107. New York: Academic Press 1975Google Scholar
  4. 4.
    Bock, E., Jørgensen., O. S., Dittmann, L., Eng, L. F.: Determination of brain-specific antigens in short term cultivated rat astroglial cells and in rat synaptosomes. J. Neurochem.25, 867–870 (1975)Google Scholar
  5. 5.
    Fields, K. L., Gosling, C., Megson, M., Stern, P. L.: New cell surface antigens in rat defined by tumors of the nervous system. Proc. nat. Acad. Sci. (Wash.)72, 1296–1300 (1975)Google Scholar
  6. 6.
    Gooding, L. R., Hsu, Y.-C., Edidin, M.: Expression of teratoma-associated antigens on murine ova and early embryos. Develop. Biol.49, 479–486 (1976)Google Scholar
  7. 7.
    Hemminki, K.: Purification of surface membranes from immature brain cells Exp. Cell Res.82, 31–38 (1973)Google Scholar
  8. 8.
    Herman, M. M., Sipe, J. C., Rubinstein, L. J., VandenBerg, S. R., Spence, A. M., Vraa-Jensen, J.: An experimental mouse testicular teratoma as a model for neuroepithelial neoplasia and differentiation. II. Electron microscopy. Amer. J. Path.81, 421–444 (1975)Google Scholar
  9. 9.
    Illmensee, K., Mintz, B.: Totipotency and normal differentiation of single teratocarcinoma cells cloned by injection into blastocysts Proc. nat. Acad. Sci. (Wash.)73, 549–553 (1976)Google Scholar
  10. 10.
    Levine, L.: Micro-complement fixation. In: Handbook of experimental immunology (ed. D. M. Weir), pp. 22.1–22.8. Oxford: Blackwell 1973Google Scholar
  11. 11.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem.193 265–275 (1951)Google Scholar
  12. 12.
    Martin, S. E.: Mouse brain antigen detected by rat anti-C1300 antiserum. Nature (Lond.)249, 71–73 (1974)Google Scholar
  13. 13.
    Orenberg, E. K., VandenBerg S. R., Barchas, J. D., Herman, M. M.: Neurochemical studies in a mouse teratoma with neuroepithelial differentiation. Presence of cyclic AMP, serotonin and enzymes of the serotonergic, adrenergic and cholinergic systems. Brain Res.101, 273–281 (1976)Google Scholar
  14. 14.
    Schachner, M.: NS-1 (nervous system antigen-1), a glialcell-specific antigenic component of the surface membrane. Proc. nat. Acad. Sci. (Wash.)71, 1795–1799 (1974)Google Scholar
  15. 15.
    Schachner, M., Carnow, T. B.: Nervous system antigen-2 (NS-2), an antigenic cell surface component expressed on a murine glioblastoma. Brain Res.88, 394–402 (1975)Google Scholar
  16. 16.
    Schachner, M., Wortham, K. A.: Nervous system antigen-3 (NS-3), an antigenic cell surface component expressed on neuroblastoma C1300. Brain Res.99, 201–208 (1975)Google Scholar
  17. 17.
    Schachner, M., Wortham, K. A., Carter, L. D., Chaffee, J. K.: NS-4 (nervous system antigen-4), a cell surface antigen of developing and adult mouse brain and sperm. Develop. Biol.44, 313–325 (1975)Google Scholar
  18. 18.
    Schachner, M., Wortham, K. A., Kincade, P. W.: Detection of nervous-system specific cells surface antigen(s) by heterologous anti-mouse brain antiserum. Cell Immunol.22, 369–374 (1976)Google Scholar
  19. 19.
    Seeds, N. W.: Cerebellar cell surface antigens of mouse brain. Proc. nat. Acad. Sci. (Wash.)72, 4110–4114 (1975)Google Scholar
  20. 20.
    Solter, D., Schachner, M.: Brain and sperm cell surface antigen (NS-4) on preimplanatation mouse embryos. Develop. Biol.52, 98–104 (1976)Google Scholar
  21. 21.
    Stern, P. L., Martin, G. R., Evans, M. J.: Cell surface antigens of clonal teratocarcinoma cells at various stages of differentiation. Cell6, 455–465 (1975)Google Scholar
  22. 22.
    Stevens, L. C.: The development of transplantable of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Develop. Biol.21 364–382 (1970)Google Scholar
  23. 23.
    Toh, B. H., Cauchi, M. N.: Brain-associated tumour antigens demonstrated by immunofluorescence. Nature (Lond.)250, 597–598 (1974)Google Scholar
  24. 24.
    Tresman, R. L., Evans, M. J.: A light and electron-microscopical study of the nervous tissue of mouse teratomas. J. Neurocytol.4, 301–314 (1975)Google Scholar
  25. 25.
    VandenBerg, S. R., Herman, M. M., Ludwin, S. K., Bignami, A.: An experimental mouse testicular teratoma as a model for neuroepithelial neoplasia and differentiation. I. Light microscopic and tissue and organ culture observations. Amer. J. Path.79, 147–168 (1975)Google Scholar
  26. 26.
    VandenBerg, S. R., Ludwin, S. K., Herman, M. M., Bignami, A.: In vitro astrocytic differentiation from embryoid bodies of an experimental mouse testicular teratoma. Amer. J. Path.83, 197–212 (1976)Google Scholar
  27. 27.
    Wahlström, T., Linder, E., Saksela, E.: Glia-specific antigens in cell cultures from rabbit brain, human foetal and adult brain, and gliomas. Acta path. microbiol. scand.81, 768–774 (1973)Google Scholar
  28. 28.
    Zimmermann, A., Schachner, M.: Nervous system antigen-5, an antigenic cell surface component of neuroectodermal origin. Brain Res.115, 297–310 (1976)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • S. R. Vanden Berg
    • 1
  • J. E. Hickey
    • 1
  • M. M. Herman
    • 1
  1. 1.Department of Pathology (Neuropathology)Stanford University School of MedicineStanfordUSA

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