Journal of comparative physiology

, Volume 117, Issue 3, pp 323–334 | Cite as

Intestinal absorption of amino acids by rainbow trout,Salmo gairdneri (Richardson)

  • L. Ingham
  • C. Arme
Article

Summary

A technique for the in vitro maintenance of isolated portions of rainbow trout intestine is described. Uptake of14C-L-leucine occurs by an active mechanism which is stereospecific, sodium-dependent and susceptible to inhibition by other neutral amino acids.K t for leucine uptake is 2.72 mM with aVmax of 19.61 μmoles/g ethanol extracted dry wt.·10 min. L-valine and L-methionine are competitive inhibitors of L-leucine uptake withK i values of 24.30 mM and 2.56 mM, respectively. Evidence suggests that at least two uptake sites for the transport of neutral amino acids are present in the intestine of this species.

Keywords

Human Physiology Leucine Rainbow Trout Active Mechanism Competitive Inhibitor 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahmed, K., Scholefield, P.: Biochemical studies on 1-aminocyclopentane carboxylic acid. Canad. J. Biochem. Physiol.40, 1101–1110 (1962)Google Scholar
  2. Akedo, H., Christensen, H.N.: Nature of insulin action on amino acid uptake by the isolated diaphragm. J. biol. Chem.237, 118–122 (1962)Google Scholar
  3. Arme, C., Ingham, L.:Proteocephalus sp. in rainbow trout,Salmo gairdneri: a new host record for the Palaearctic. Ir. Nat. J.17, 241–242 (1972)Google Scholar
  4. Bailey, N.T.: Statistical methods in biology. London: English Universities Press Ltd. 1959Google Scholar
  5. Bamford, D.R.: Studiesin vitro of the passage of serum proteins across the gut-walls of young rats. Ph.D. Thesis, University College of North Wales, Bangor, North Wales (1965)Google Scholar
  6. Fisher, R.B., Parsons, D.S.: Glucose movements across the wall of the rat small intestine. J. Physiol. (Lond.)119, 210–223 (1953)Google Scholar
  7. Huang, K.C., Rout, W.R.: Intestinal transport of sugars and aromatic amino acids in killifish,Fundulus heteroclitus. Amer. J. Physiol.212, 799–803 (1967)Google Scholar
  8. Huang, K.C., Rout, W.R., Lin, S.R.: Intestinal transport of sugars and aromatic amino acids in marine fish and selected fresh water fish. Bull. Mount Desert Island Biol. Lab.5, No. 21 (1965)Google Scholar
  9. Ingham, L., Arme, C.: Intestinal helminths in rainbow trout,Salmo gairdneri: Absence of effect on nutrient absorption and fish growth. J. Fish. Biol.5, 309–313 (1973)Google Scholar
  10. Kemp, P., Smith, M.W.: Effect of temperature acclimatisation on the fatty acid composition of goldfish intestinal lipids. Biochem. J.117, 9–17 (1970)Google Scholar
  11. Kitchin, S.E., Morris, D.: The effect of acclimation temperature on amino acid transport in the goldfish intestine. Comp. Biochem. Physiol.40A, 431–443 (1971)Google Scholar
  12. Lineweaver, H., Burk, D.: The determination of enzyme dissociation constants. J. Amer. chem. Soc.56, 658–666 (1934)Google Scholar
  13. Mepham, T.B., Smith, M.W.: Amino acid transport in the goldfish intestine. J. Physiol. (Lond.)184, 673–684 (1966a)Google Scholar
  14. Mepham, T.B., Smith, M.W.: Regulation of amino acid transport across intestines of goldfish acclimatised to different environmental temperatures. J. Physiol. (Lond.)186, 619–631 (1966b)Google Scholar
  15. Miller, D.S., Burrell, P., Lerner, J.: Distinct components of neutral amino acid transport in chick small intestine. Comp. Biochem. Physiol.47A, 767–777 (1974)Google Scholar
  16. Musacchia, X.J., Fisher, S.S., Westhoff, D.: Uptake and transport of L-leucine by intestinal segments ofAmeiurus nebulosus andStenotomus versicolor. Biol. Bull.121, 400 (1961)Google Scholar
  17. Neame, K.D., Richards, T.G.: Elementary kinetics of membrane carrier transport. Oxford: Blackwell Scientific Publications 1972Google Scholar
  18. Neff, S.S., Musacchia, X.J.: Intestinal absorption of L-leucinein vitro in fish (Stenotomus versicolor andIctalurus nebulosus). Comp. Biochem. Physiol.21, 337–343 (1967)Google Scholar
  19. Read, C.P.: Studies on membrane transport I. A common transport system for sugars and amino acids? Biol. Bull.133, 630–642 (1967)Google Scholar
  20. Read, C.P., Simmons, J.E., Jr., Campbell, J.W., Rothman, A.H.: Permeation and membrane transport in parasitism: studies on a tapeworm-elasmobranch symbiosis. Biol. Bull.119, 120–133 (1960)Google Scholar
  21. Rout, W.R., Lin, D.S.T., Huang, K.C.: Intestinal transport of amino acids and glucose in flounder fish. Proc. Soc. exp. Biol. (N.Y.)118, 933–938 (1965)Google Scholar
  22. Schultz, S.G., Curran, P.F.: Coupled transport of sodium and organic solutes. Physiol. Revs.50, 637–718 (1970)Google Scholar
  23. Smith, M.W.: Selective regulation of amino acid transport by the intestine of goldfish. Comp. Biochem. Physiol.35, 387–401 (1970)Google Scholar
  24. Smith, M.W., Ellory, J.C.: Sodium-amino acid interactions in the intestinal epithelium. Phil. Trans. B262, 131–140 (1971)Google Scholar
  25. Smith, M.W., Kemp, P.: Parallel temperature-induced changes in membrane fatty acids and in the transport of amino acids by the intestine of goldfish (Carassius auratus L.). Comp. Biochem. Physiol.39B, 357–365 (1971)Google Scholar
  26. Smith, R.L.: Intestinal amino acid transport in the marine teleost,Haemulon plumieri. Comp. Biochem. Physiol.30, 1115–1123 (1969)Google Scholar
  27. Snecedor, G.W., Cochran, W.G.: Stastical methods. Ames, Iowa: Iowa State University Press 1967Google Scholar
  28. Stokes, R.M., Fromm, P.O.: Glucose absorption and metabolism by the gut of rainbow trout. Comp. Biochem. Physiol.13, 53–69 (1964)Google Scholar
  29. Warburg, O.: Versuche an überlebendem Carcinomgewebe (Methoden). Biochem. Z.142, 317–333 (1923)Google Scholar
  30. Ward, W.E.: The uptake of amino acids by intestinal segments of the trout,Salmo gairdneri Ph.D. Thesis, College of Arts and Sciences, Kent State University. Ohio, U.S.A. (1968)Google Scholar
  31. Wilson, T.H.:In vitro studies on intestinal absorption of fish. Biol. Bull.113, 362 (1957)Google Scholar
  32. Wilson, T.H.: Intestinal absorption. Philadelphia and London: W.B. Saunders Co. 1962Google Scholar
  33. Wilson, T.H., Wiseman, G.: Metabolic activity of the small intestine of the rat and golden hamster (Mesocricetus auratus). J. Physiol. (Lond.)127, 414–422 (1954)Google Scholar
  34. Wiseman, G.: Absorption of amino acids. In: Handbook of physiology, Sect. 6, Alimentary canal, Vol. 3.: Intestinal absorption (ed. C.F. Code), pp. 1277–1307. Washington, D.C.: American Physiological Society 1968Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • L. Ingham
    • 1
  • C. Arme
    • 1
  1. 1.Department of ZoologyQueen's UniversityBelfastNorthern Ireland

Personalised recommendations