Journal of Comparative Physiology B

, Volume 159, Issue 3, pp 339–347 | Cite as

Presence of a Na+/H+ exchanger in brush border membranes isolated from the kidney of the spiny dogfish,Squalus acanthias

  • C. Bevan
  • R. K. H. Kinne
  • R. E. Shetlar
  • E. Kinne-Saffran


A membrane fraction, rich in brush border membranes, was prepared from renal proximal tubules of the spiny dogfish,Squalus acanthias, and the sodium-proton exchange mechanism in these membrane vesicles was investigated by both a rapid filtration technique and the fluorescence quenching of acridine organe.22Na+ uptake was stimulated by an outwardly directed H+ gradient, and was inhibited by amiloride at a single inhibitory site with an apparentK i of approximately 1.7×10−5M. In the presence of an H i + >H o + gradient, the\(K_{{\text{m}}_{{\text{Na}}} + } {\text{and}} V_{\max _{{\text{Na}}} + } \) of the Na+/H+ exchanger were 9.7±0.8 mM and 48.0±12.0 nmol·mg protein−1·min−1, respectively. The uptake of Na+ was electroneutral in the presence of a H+ gradient, indicating a stoichiometry of 1. In the fluorescence studies, quenching of acridine orange occurred in the presence of an outwardly directed Na+ gradient which was inhibited by amiloride. Thus, an electroneutral Na+/H+ exchanger with properties similar to those found in the mammalian kidney is also present in the spiny dogfish and may contribute to the urinary acidification of this marine animal.

Key words

Renal acidification Na+/H+ exchange Proximal tubule Amiloride Brush border membrane vesicles Squalus acanthias 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • C. Bevan
    • 1
    • 2
  • R. K. H. Kinne
    • 1
    • 2
  • R. E. Shetlar
    • 1
    • 2
  • E. Kinne-Saffran
    • 1
    • 2
  1. 1.Max-Planck-Institut für SystemphysiologieDortmund 1Federal Republic of Germany
  2. 2.Mount Desert Island Biological LaboratorySalsbury CoveUSA

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