Journal of Comparative Physiology B

, Volume 159, Issue 3, pp 287–292 | Cite as

Calcium movements associated with peptide induced phagocytosis inAmoeba proteus

  • Robert D. Prusch
  • Philip A. Vedovatti
Article

Summary

Calcium controls the level of phagocytosis inAmoeba proteus which is inhibited by substances such as verapamil and flunarazine which interfere with Ca++ movements in a variety of cell systems. Calcium ion movements in amoebae under control conditions appear to be primarily diffusive in response to activity and electrical potential gradients. Chemotactic peptides (n-formylmethionylleucylphenylalanine, NFMLP) at high concentrations (10−5M) induce phagocytosis in the amoeba and bring about a concomitant increase in45Ca influx. Verapamil, flunarazine and La+++ all block the increased45Ca influx caused by NFMLP, as well as inhibiting phagocytosis. It is suggested that initiation of phagocytosis in the amoeba is associated with the movement of Ca++ into the cytoplasm from the external medium resulting in a transient increase in the cytoplasmic Ca++ ion activity.

Key words

Amoeba proteus Phagocytosis Calcium flux Peptide 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Robert D. Prusch
    • 1
  • Philip A. Vedovatti
    • 1
  1. 1.Department of Life SciencesGonzaga UniversitySpokaneUSA

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