Acta Neuropathologica

, Volume 4, Issue 6, pp 590–603 | Cite as

Electron microscopy of focal neuroaxonal lesions produced by β-β′-iminodipropionitrile (IDPN) in rats

I. The advanced lesions
  • Shi-Ming Chou
  • Henrik A. Hartmann
Original Investigations

Summary and Conclusions

An electron microscopic study was made on neuroaxonal lesions in the anterior motoneurons of the lumbar spinal cord in IDPN-treated rats which manifested the permanent “waltzing syndrome” during the stages of axonal balloon formation. The following observations and discussion were made.
  1. 1.

    Within the axonal balloons, there were focal accumulations of usual axoplasmic constituents, viz., neurofibrils, mitochondria, and small vesicles.

     
  2. 2.

    The balloons, outlined by a thin myelin or axolemmal layer, or axoplasmic contents without definite membrane structure, were often surrounded by edematous spaces filled with granular material.

     
  3. 3.

    Distal to the balloons, there were segmental accumulations of degenerating mitochondria, vesicles, and also of dense complex bodies within the myelinated portions of axons.

     
  4. 4.

    No significant alterations were observed in the perikarya of the anterior motoneurons.

     
  5. 5.

    The present study seems to support a previously held hypothesis of a mechanism of “primary axostasis” as the cause of the lesions as well as of the waltzing syndrome. The intra-axonic dense complex bodies, resembling pigment granules, would provide a plausible explanation for the permanence of the induced syndrome.

     

Keywords

Spinal Cord Granular Material Electron Microscopic Study Membrane Structure Small Vesicle 

Zusammenfassung

Elektronenmikroskopische Untersuchungen der neuroaxonalen Läsionen an den Vorderhornzellen des Lumbalmarks von IDPN-behandelten Ratten mit permanentem “waltzing syndrome” wurden während des Stadiums der axonalen Ballonierung durchgeführt. Es wurden folgende Beobachtungen gemacht und diskutiert:
  1. 1.

    Innerhalb der Axonballons finden sich fokale Anhäufungen üblicher Axoplasmabestandteile, z. B. Neurofibrillen, Mitochondrien und kleine Vesikeln.

     
  2. 2.

    Die Ballons, die von einer dünnen Myelin-oder Axolemmschicht oder von Axoplasmaanteilen ohne distinkte Membranstruktur begrenzt sind, werden oft von Ödemräumen umgeben, die von granulärem Material erfüllt sind.

     
  3. 3.

    Distal von den Ballons findet sich eine segmentale Anhäufung von degenerierten Mitochondrien, Vesikeln sowie von dichten zusammengesetzten Körperchen innerhalb der bemarkten Abschnitte des Axons.

     
  4. 4.

    In den Perikarya der Vorderhornzellen finden sich keine nennenswerten Veränderungen.

     
  5. 5.

    Die vorliegende Untersuchung scheint die frühere Hypothese des Mechanismus der “primären Axostase” als Ursache der Läsionen beim “waltzing syndrom” zu unterstützen. Die intraaxonalen dichten zuxammengesetzten Körper dürften eine plausible Erklärung für die Permanenz des induzierten Syndroms geben.

     

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Copyright information

© Springer-Verlag 1965

Authors and Affiliations

  • Shi-Ming Chou
    • 1
  • Henrik A. Hartmann
    • 1
  1. 1.Department of PathologyUniversity of Wisconsin Medical SchoolMadisonUSA

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