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Archives of Microbiology

, Volume 132, Issue 1, pp 96–99 | Cite as

Continuous culture and synchronization ofHyphomicrobium sp. B-522

  • N. Matzen
  • P. Hirsch
Short Communications

Abstract

A technique was developed for synchronization ofHyphomicrobium sp. strain B-522. Bacteria were grown in continuous culture with methanol (0.1%; v/v) growth limiting. Vitamin B12 (2.5 μg/l) was necessary to obtain steady state growth. The critical dilution rate wasD c =0.112; maximum cell output occurred atD=0.105 (Dx=30 mg l-1 h-1). Continuous cultures ofHyphomicrobium B-522 atD=0.110 were used to obtain cells for synchronization experiments. Synchronization was achieved by trapping young hyphal and budding cells in a glass wool column, while the initial swarmer cells were allowed to pass through. By semicontinuously rinsing the system, newly produced swarmers could be sampled in the effluent. The mean length of these synchronous swarmer cells was 1.25 μm (s=±0.13 μm; range 0,6 μm) as compared to 1.40 μm (s=±0.21 μm; range 1.2 μm) for swarmer cells of the continuous culture. Division of synchronous swarmer populations was completed after 7 h; the synchronization index was 0.76.

Key words

Hyphomicrobium Continuous culture Synchronization technique Synchronous swarmer cells 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • N. Matzen
    • 1
  • P. Hirsch
    • 1
  1. 1.Institut für Allgemeine MikrobiologieUniversität KielKielGermany

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