Abstract
Protoplasts prepared from complementary haploid strains ofSaccharomyces cerevisiae were studied with regard to their ability of conjugating. Neither fresh protoplasts nor the growing protoplasts possessing fibrillar walls exhibited sex specific agglutination or fusion. However, they were capable of inducing sexual activation in normal cells of opposite mating type. After completing the regeneration of cell walls the protoplasts could conjugate either with each other or with cells of opposite sex. The frequency of conjugations was low, about 1%, and was largely dependent on the degree of completition of the wall during regeneration. From the results the following conclusions may be drawn: 1. The initiation of mating is dependent on the integrity of the cell wall. 2. The sex specific morphogenetic changes do not occur in wall-less protoplasts but may happen after the protoplasts have regenerated their cell walls. 3. The lysis of cell walls does not occur until the walls come into close contact. 4. The fusion of plasma membranes in sex-activated protoplasts cannot be induced by artefucial agglutination.
Similar content being viewed by others
References
Crandall, M., Brock, T. D.: Molecular basis of mating in the yeastHansenula winger. Bact. Rev.32, 139–163 (1968)
Duntze, W., MacKay, V., Manney, T. R.:Saccharomyces cerevisiae: A diffusible sex factor. Science168, 1472–1473 (1970)
Eddy, A. A., Williamson, D. H.: Formation of aberrant cell walls and of spores by the growing yeast protoplasts. Nature (Lond.)183, 1101–1104 (1959)
Fowell, R. R.: Sporulation and hybridization of yeast. In: The yeasts, Vol. 1 (A. H. Rose, J. S. Harrison, eds.), pp. 303–383. London-New York: Academic Press 1969
Hartwell, L. H.: Synchronization of haploid yeast cell cycles, a prelude to conjugation. Exp. Cell Res.76, 111–117 (1973)
Koch, Y., Koch, H. A.: Der intranucleare Spindelapparat bei Hefeprotoplasten. Z. allg. Mikrobiol.14, 535–537 (1974)
Kreger, D. R., Kopecká, M.: On the nature of the fibrillar nets formed by protoplasts ofSaccharomyces cerevisiae in liquid media. In: Yeast, mould and plant protoplasts (J. R. Villanueva, I. Garcia-Acha, F. Uruburu, S. Gascón, eds.), pp. 117–130. London-New York: Academic Press 1973
Nečas, O.: The mechanism of regeneration of yeast protoplasts. I. Physical conditions. Folia biol. (Praha)8, 256–262 (1962)
Nečas, O.: The mechanism of regeneration of yeast protoplasts. II. Formation of the cell wall de novo. Folia biol. (Praha)11, 97–102 (1965)
Nečas, O.: Cell wall synthesis in yeast protoplasts. Bact. Rev.35, 149–170 (1971)
Nečas, O., Svoboda, A.: The mechanism of regeneration of yeast protoplasts. IV. Electron microscopy of regenerating protoplasts. Folia biol. (Praha)3, 379–385 (1967)
Osumi, M., Shimoda, C., Yanagishima, N.: Mating reaction inSaccharomyces cerevisiae. V. Changes in the fine structure during the mating reaction. Arch. Microbiol.97, 27–38 (1974)
Sakai, K., Yanagishima, N.: Mating reaction inSaccharomyces cerevisiae. I. Cell agglutination related to mating. Arch. Mikrobiol.75, 260–265 (1971)
Sena, E. P., Radin, D. N., Fogel, S.: Synchronous mating in yeast. Proc. nat. Acad. Sci. (Wash.)70, 1373–1377 (1973)
Streiblová, E.: Study of scar formation in the life cycle of heterothalicSaccharomyces cerevisiae. Canad. J. Microbiol.16, 827–831 (1970)
Streiblová, E., Wolf, A.: Role of cell wall topography in conjugation ofSchizosaccharomyces pombe. Canad. J. Microbiol.21, 1399–1405 (1975)
Svoboda, A.: Regeneration of yeast protoplasts in agar gels. Exp. Cell Res.44, 640–642 (1966)
Wickerham, L. J.: Taxonomy of yeast. US Dept. Agr. Techn. Bull. No. 1029 (1951)
Yanagishima, N.: Physiology of the mating reaction in yeasts. Curr. Advance Plant Sci.7, 55–66 (1973)
Yen, P. H., Ballou, C. E.: Composition of a specific intercellular agglutination factor. J. biol. Chem.248, 8316–8318 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Svoboda, A. Mating reaction in yeast protoplasts. Arch. Microbiol. 110, 313–318 (1976). https://doi.org/10.1007/BF00690244
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00690244