Abstract
A glycerol-requiring mutant ofBacillus subtilis formed irregular spheres and showed disturbed septum formation, when subjected to growth limitation by the supply of glycerol. Under phosphate limitation the cells were also round and developed asymmetric septa. In magnesium-limited cultures the cells contained a thickened wall, as compared with that of the parent strain grown under the same conditions. Chemical analysis revealed the presence of teichoic acid as the major anionic polymer in the wall of the glycerol-, as well as the magnesium-limited cells of the glycerol-requiringB. subtilis mutant.
Under phosphate limitation teichuronic acid was the only anionic polymer present in the wall. Thus, in this respect, there were no apparent differences between mutant organisms and the parent strain when grown under magnesium and phosphate limitation, respectively and the observed morphological deviations could not be correlated with an altered anionic polymer content of the wall.
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Ames, B. H., Dubin, D. T.: The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J. biol. Chem.235, 769–775 (1960)
Ashwell, G.: Colorimetric analysis of sugars. In: Methods in enzymology. Vol. 3 (S. P. Colowick, N. O. Kaplan, eds.), pp. 73–105. New York: Academic Press 1957
de Boer, W. R., Kruyssen, F. J., Wouters, J. T. M., Kruk, C.: The structure of teichoic acid fromBacillus subtilis var.niger WM as determined by13C Nuclear-Magnetic-Resonance spectroscopy. Europ. J. Biochem.62, 1–6 (1976)
Brown, W. C., Wilson, C. R., Lukehart, S., Young, F. E., Shiflett, M. A.: Analysis of autolysins in temperature-sensitive morphological mutants ofBacillus subtilis. J. Bact.125, 166–173 (1976)
Cáslavská, J., Kodesová, J., Horáková, I.: Effect of prolonged cultivation in a chemostat under nitrogen limitation on the morphology and ultrastructure ofBacillus subtilis. Folia microbiol. (Praha)17, 126–131 (1972)
Ellwood D. C., Tempest, D. W.: Control of teichoic acid and teichuronic acid biosyntheses in chemostat cultures ofBacillus subtilis var.niger. Biochem. J.111, 1–5 (1969)
Evans, C. G. T., Herbert, D., Tempest, D. W.: The continuous cultivation of micro-organisms. 2. Construction of a chemostat. In: Methods in microbiology, Vol. 2 (I. R. Norris, D. W. Ribbons, eds.), pp. 310–313. New York: Academic Press 1970
Fan, D. P., Beckman, M. M. Cunningham, W.P.: Ultrastructural studies on a mutant ofBacillus subtilis whose growth is inhibited due to insufficient autolysin production. J. Bact.109, 1247–1257 (1972)
Forsberg, C. W., Wyrick, P. B., Ward, J. B., Rogers, H. J.: Effect of phosphate limitation on the morphology, and wall composition ofBacillus licheniformis and its phosphoglucomutase-deficient mutants. J. Bact.113, 969–984 (1973)
Freese, E. B., Oh, Y. K.: Adenosine 5′-triphosphate release and membrane collapse in glycerol-requiring mutants ofBacillus subtilis. J. Bact.120, 507–515 (1974)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951)
Mindich, L.: Membrane synthesis inBacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J. molec. Biol.49, 415–432 (1970a)
Mindich, L.: Membrane synthesis inBacillus subtilis. II. Integration of membrane proteins in the absence of lipid synthesis. J. molec. Biol.49, 433–439 (1970b)
Minnikin, D. E., Abdolrahimzadeh, H.: Effect of pH on the proportions of polar lipids, in chemostat cultures ofBacillus subtilis. J. Bact.120, 999–1003 (1974)
Oh, Y. K., Freese, E. B.: Abnormal septation, and inhibition of sporulation by accumulation ofl-α-glycerophosphate inBacillus subtilis mutants. J. Bact.113, 1034–1045 (1973)
Ryter, A., Kellenberger, E., Birch-Andersen, A., Maaløe, O.: Étude au microscope électronique de plasmas contenant de l'acide désoxyribonucléique. I. Les nucléoides des bactéries en croissance active. Z. Naturforsch.13B 597–605 (1958)
Tempest D. W., Dicks, J. W., Ellwood, D. C.: Influence of growth condition on the concentration of potassium inBacillus subtilis var.niger and its possible relationship to cellular ribonuclear acid, teichoic acid and teichuronic acid. Biochem. J.106, 237–243 (1968)
Wardi, A. H., Allen, W. S., Varma, R.: A simple method for the detection and quantitative determination of hexuronic acids and pentoses. Analyt. Biochem.57, 268–273 (1974)
Wicland, O.: Glycerin UV method. In: Methoden der enzymatischen Analyse. Bd. II (H. M. Bergmeyer, Hrsg.) 1448–1453. Weinheim: Verlag Chemie 1974
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Wouters, J.T.M., Leegwater, M.P.M. Morphology and anionic polymer content in the cell wall of a glycerol-requiring mutant ofBacillus subtilis . Arch. Microbiol. 110, 295–300 (1976). https://doi.org/10.1007/BF00690241
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DOI: https://doi.org/10.1007/BF00690241