Archives of Microbiology

, Volume 110, Issue 2–3, pp 287–293 | Cite as

d-Alanine dehydrogenase

Its role in the utilisation of alanine isomers as growth substrates byPseudomonas aeruginosa PA01
  • D. Pioli
  • W. A. Venables
  • F. C. H. Franklin
Article

Abstract

Pseudomonas aeruginosa PA01 was found to utilise both thed- andl-isomers of α-alanine and also β-alanine as sole sources of carbon and energy for growth. Enzymological studies of wild-type cultures and comparison with mutants deficient in growth upon one or more isomers of alanine led to the following conclusions: (i) utilisation ofd-alanine involved its direct oxidation by an inducible, membrane-bound, cytochrome-linked dehydrogenase; (ii) utilisation ofl-alanine required its conversion to the directly oxidisabled-form by a soluble racemase; (iii) utilisation of β-alanine, likel-alanine, involves both the racemase andd-alanine dehydrogenase enzymes, but in addition must involve other enzymes the identity, of which is still speculative; (iv)P. aeruginosa, likeEscherichia coli, appears to take upd-alanine andl-alanine by means of two specific permeases.

Key words

d-Alanine l-Alanine β-Alanine Dehydrogenase Oxidase Pseudomonas aeruginosa Catabolism 

Abbreviation

DCPIP

2,6-dichlorophenol-indophenol

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • D. Pioli
    • 1
  • W. A. Venables
    • 1
  • F. C. H. Franklin
    • 1
  1. 1.Department of MicrobiologyUniversity CollegeCardiffEngland

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