Abstract
Uredospores of a barley isolate ofPuccinia striiformis West. were germinated on glucose peptone agar and the development of colonies was observed with a microscope. A small number (0.1–1%) of germ tubes did not abort during the first 20 h of incubation and either differentiated an infection structure or continued slowly to elongate for several days. In 4 out of 9 experiments some of these sporelings produced colonies that reached maximum size (0.15–0.20 mm) after 3 weeks at 15° C.
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References
Bose, A., Shaw, M.: In vitro growth of wheat and flax rust fungi on complex and chemically defined media. Canad. J. Bot.52, 1183–1195 (1974)
Coffey, M. D., Allen, P. J.: Nutrition ofMelampsora lini andPuccinia helianthi. Trans. Brit. Mycol. Soc.60, 245–260 (1973)
Foudin, A. S., Wynn, W. K.: Growth ofPuccinia graminis f. sp.tritici on a defined medium. Phytopathology62, 1032–1040 (1972)
Fuchs, W. H., Gaertner, A.: Untersuchungen zur Keimungs-physiologie des SchwarzrostesPuccinia graminis tritici (Pers.) Erickss. u. Henn. Arch. Mikrobiol.28, 303–309 (1958)
Hartley, M. J., Williams, P. G.: Morphological and cultural differences between races ofPuccinia graminis f. sp.tritici in axenic culture. Trans. Brit. Mycol. Soc.57, 137–144 (1971)
Hassebrauk, K.: Der GelbrostPuccinia striiformis. West. II. Befallsbild. Morphologie und Biologie der Sporen. Infektion und weitere Entwicklung. Wirkung auf die Wirtspflanze. Mitt. Biol. Bundesanst.139 (1970)
Jones, D. R.: In vitro culture of carnation rust,Uromyces dianthi. Trans. Brit. Mycol. Soc.58, 29–36 (1972)
Kuhl, J.L., Maclean, D.J., Scott, K.J., Williams, P.G.: The axenic culture ofPuccinia species from uredospores: Experiments on nutrition and variation. Canad. J. Bot.49, 201–209 (1971)
Macko, V., Fuchs, E.: Effect of carbon dioxide on uredospore germ tubes ofPuccinia striiformis. Phytopathology60, 1529–1530 (1970)
Straib, W.: Zur Kenntnis des Keimschlauchwachstums der Uredosporen einiger Getreiderostarten und ihrer Rassen. Ber. dtsch. bot. Ges.57, 136–154 (1939)
Williams, P. G., Scott, K. J., Kuhl, J. L.: Vegetative growth ofPuccinia graminis f. sp.tritici in vitro. Phytopathology56, 1418–1419 (1966)
Williams, P. G.: A new perspective of the axenic culture ofPuccinia graminis f. sp.tritici from uredospores. Phytopathology61, 994–1002 (1971)
Wolf, F. T.: The cultivation of rust fungi upon artificial media. Canad. J. Bot.52, 767–772 (1974)
Wong, A.L., Willetts, H. J.: Observations on the growth of selected Australian races of wheat stem rust in axenic culture. Trans. Brit. Mycol. Soc.55, 231–238 (1970)
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Williams, P.G. Development ofPuccinia striiformis West. on nutrient agar. Arch. Microbiol. 110, 173–175 (1976). https://doi.org/10.1007/BF00690225
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DOI: https://doi.org/10.1007/BF00690225