Breast Cancer Research and Treatment

, Volume 36, Issue 1, pp 93–97 | Cite as

Prognostic significance of heat-shock protein-27 in node-positive breast carcinoma: an immunohistochemical study

  • Bernard Têtu
  • Jacques Brisson
  • Jacques Landry
  • Jacques Huot
Brief communication

Abstract

Immunostaining for heat-shock protein-27 (HSP-27) was performed on formalin fixed-paraffin embedded sections of 890 node-positive breast carcinomas resected between 1980 and 1986. The follow-up ranged from 2.5 to 10.5 years. A polyclonal antibody (Hu27, dilution: 1/200) was used. A positive cytoplasmic staining was obtained in 383 cases (43%). No difference in distant metastasis-free survival (DMFS) or overall survival (OS) was noted between cases with positive or negative immunostaining. This study suggests that HSP-27 expression is not predictive of the outcome in node-positive breast cancer.

Key words

breast cancer heat-shock prognostic factors stress-related protein 

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References

  1. 1.
    Edwards DP, Adams DJ, McGuire WL: Specific protein synthesis regulated by estrogens in breast cancer. J Steroid Biochem 15: 247–259, 1989Google Scholar
  2. 2.
    Adams DJ, McGuire WL: Quantitative enzyme-linked immunosorbent assay for the estrogen-regulated Mr 24,000 protein in human breast tumors: correlation with estrogen and progesterone receptors. Cancer Res 45: 2445–2449, 1985Google Scholar
  3. 3.
    Ciocca DR, Adams DJ, Edwards DP, Bjercke RJ, McGuire WL: Distribution of an estrogen-induced protein with a molecular weight of 24,000 in normal and malignant human tissues and cells. Cancer Res 43: 1204–1210, 1983Google Scholar
  4. 4.
    Ciocca DR, Puy LA, Fasoli LC: Study of estrogen receptor, progesterone receptor, and the estrogen-regulated Mr 24,000 protein in patients with carcinomas of the endometrium and cervix. Cancer Res 49: 4298–4304, 1989Google Scholar
  5. 5.
    Thor A, Benz C, Moore D II, Goldman E, Edgerton S, Landry L, Schwartz L, Mayall B, Hickey E, Weber LA: Stress response protein (srp-27) determination in primary human breast carcinomas: clinical, histologic, and prognostic correlations. J Nat Cancer Inst 83: 170–178, 1991Google Scholar
  6. 6.
    Têtu B, Lacasse B, Bouchard H-L, Lagacé R, Huot J, Landry J: Prognostic influence of HSP-27 expression in malignant fibrous histiocytoma: a clinicopathological and immunohistochemical study. Cancer Res 52: 2325–2328, 1992Google Scholar
  7. 7.
    Navarro D, Cabrera LL, Leon L, Chirino R, Fernandez L, Lopez A, Rivero JF, Fernandez P, Falcon O, Jimenez P, Pestano J, Diaz-Chico JC, Diaz-Chico BN: Endometrial stromal sarcoma expression of estrogen receptors, progesterone receptors and estrogen-induced srp27 (24 K) suggests hormone responsiveness. J Steroid Biochem Mol Biol 41: 589–596, 1992Google Scholar
  8. 8.
    Landry J, Chrétien P, Lambert H, Hickey E, Weber LE: Heat-shock resistance conferred by expression of the human HSP-27 gene on rodent cells. J Cell Biol 109: 7–15, 1989Google Scholar
  9. 9.
    Huot J, Roy G, Lambert H, Chrétien P, Landry J: Increased survival after treatments with anticancer agents in Chinese hamster cells expressing the human Mr 27,000 heat-shock protein. Cancer Res 51: 5245–5252, 1991Google Scholar
  10. 10.
    Ciocca DR, Fuqua SA, Lock Lim S, Toft DO, Welch WJ, McGuire WL: Response of human breast cancer cells to heat-shock and chemotherapeutic drugs. Cancer Res 52: 3648–3654, 1992Google Scholar
  11. 11.
    Tandon AK, Clark GM, Chamness GC, Fuqua SA, Allred DC, Welch WJ, Riehl RM, McGuire WL: Clinical significance of heat-shock/stress response protein in breast cancer (abstract 6). Breast Cancer Res Treat 16: 146, 1990Google Scholar
  12. 12.
    Seymour L, Bezwoda WR, Meyer K: Tumor factors predicting for prognosis in metastatic breast cancer. Cancer 66: 2390–2394, 1990Google Scholar
  13. 13.
    Fisher ER, Sass R, Fisher B, and collaborating NSABP investigators: Pathologic findings from the National Surgical Adjuvant Project for breast cancers (Protocol No. 4). Cancer 53: 712–723, 1984Google Scholar
  14. 14.
    Hsu S-M, Raine L, Fanger H: Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques. A comparison between ABC and unlabeled antibody (ABC) procedures. J Histochem Cytochem 29: 577–580, 1981Google Scholar
  15. 15.
    Dressler LG, Bartow SA: DNA flow cytometry in solid tumors: practical aspects and clinical applications. Sem Diagn Pathol 6: 55–82, 1989Google Scholar
  16. 16.
    Kaplan EL, Meier P: Non-parametric estimation from incomplete observations. J Amer Statist Assoc 53: 453–481, 1958Google Scholar
  17. 17.
    Armitage P: Statistical Methods in Medical Research. New York: John Wiley & Sons, 1991Google Scholar
  18. 18.
    Cox DR: Regression models and lifetables (with discussion). J R Statist Soc 34: 187–220, 1972Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Bernard Têtu
    • 1
  • Jacques Brisson
    • 2
  • Jacques Landry
    • 1
  • Jacques Huot
    • 1
  1. 1.Department of Pathology and ‘Le Centre de Recherche en Cancérologie de l'Université Laval’L'Hôtel-Dieu de QuébecQuébecCanada
  2. 2.Groupe de Recherche en Épidémiologie de l'Université LavalHôpital du St-SacrementQuébecCanada

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