Abstract
Isolated rigid layers fromProteus mirabilis harvested at different growth phases were degraded by endo-N-acetylmuramidase fromChalaropsis B, and the degradation products were investigated. The results show the complete absence of covalently linked lipoprotein in exponential-phase cultures. Stationary cells, however, possess covalently linked lipoprotein in amounts similar to those found inEscherichia coli orSalmonella typhimurium during all growth phases. The overall peptidoglycan structure did not change during transition from logarithmic to stationary growth. Implications of these findings for the organization of the outer membrane are discussed.
Similar content being viewed by others
References
Braun, V.: Covalent lipoprotein from the outer membrane ofEscherichia coli. Biochim. Biophys. Acta (Amst.)415, 335–377 (1975)
Braun, V., Rehn, K.: Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of theE. coli cell wall. Eur. J. Biochem.10, 426–438 (1969)
Braun, V., Rehn, K., Wolff, H.: Supramolecular structure of the rigid layer of the cell wall ofSalmonella, Serratia, Proteus, andPseudomonas fluorescens. Number of lipoprotein molecules in a membrane layer. Biochemistry9, 5041–5049 (1970)
Fleck, J., Mock, M., Minck, R., Ghuysen, J. M.: The cell envelope inProteus vulgaris P 18. Isolation and characterization of the peptidoglycan component. Biochem. Biophys. Acta (Amst.)233, 489–503 (1971)
Gmeiner, J., Kroll, H.-P., Martin, H. H.: The covalent rigid-layer lipoprotein in cell walls ofProteus mirabilis. Eur. J. Biochem.83, 227–233 (1978)
Gmeiner, J., Schlecht, S.: Molecular organization of the outer membrane ofSalmonella typhimurium. Eur. J. Biochem.93, 609–620 (1979)
Gruss, P., Gmeiner, J., Martin, H. H.: Amino-acid composition of the covalent rigid-layer lipoprotein in cell walls ofProteus mirabilis. Eur. J. Biochem.57, 411–414 (1975)
Hirota, Y., Suzuki, H., Nishimura, Y., Yasuda, S.: On the process of cellular division inEscherichia coli — Mutant ofE. coli lacking a murein-lipoprotein. Proc. Natl. Acad. Sci (Wash.)74, 1417–1420 (1977)
Katz, E., Loring, D., Inouye, S., Inouye, M.: Lipoprotein fromProteus mirabilis. J. Bacteriol.134, 674–676 (1978)
Martin, H. H.: Composition of the mucopolymer in cell walls of the unstable and stablel-form ofProteus mirabilis. J. Gen. Microbiol.36, 441–450 (1964)
Martin, H. H., Maskos, C., Burger, R.:d-Alanyl-d-alanine carboxypeptidase in the bacterial form andl-form ofProteus mirabilis. Eur. J. Biochem.55, 465–473 (1975)
Martin, H. H., Gmeiner, J.: Modification of peptidoglycan structure by penicillin action in cell walls ofProteus mirabilis. Eur. J. Biochem. in press 1979
Torti, S. V., Park, J. T.: Lipoprotein of gram-negative bacteria is essential for growth and division. Nature (Lond.)263, 323–326 (1976)
Weigand, R. A., Vinci, K. D., Rothfield, L. I.: Morphogenesis of the bacterial division septum: A new class of septation-defective mutants. Proc. Natl. Acad. Sci. (Wash.)73, 1882–1886 (1976)
Yamada, H., Mizushima, S.: Reconstitution of an ordered structure from major outer membrane constituents and the lipoprotein-bearing peptidoglycan sacculus ofEscherichia coli. J. Bacteriol.135, 1024–1031 (1978)
Yem, D. W., Wu, H. C.: Physiological characterization of anEscherichia coli mutant altered in the structure of murein lipoprotein. J. Bacteriol.133, 1419–1426 (1978)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gmeiner, J. Covalent linkage of lipoprotein to peptidoglycan is not essential for outer membrane stability inProteus mirabilis . Arch. Microbiol. 121, 177–180 (1979). https://doi.org/10.1007/BF00689983
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00689983