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Methanogenium, a new genus of marine methanogenic bacteria, and characterization ofMethanogenium cariaci sp. nov. andMethanogenium marisnigri sp. nov.

Abstract

A new genus of marine methanogenic bacteria and two species within this genus are described.Methanogenium is the proposed genus andMethanogenium cariaci the type species. Cells of the type species are Gram-negative, peritrichously flagellated, irregular cocci with a periodic wall surface pattern. Colonies formed by these bacteria are yellow, circular and umbonate with entire edges. The DNA base composition is 52 mol% guanine plus cytosine. Formate or hydrogen and carbon dioxide serve as substrates for growth. Cells ofMethanogenium marisnigri are of similar shape but smaller diameter thanM. cariaci. The colonies ofM. marisnigri are convex, and the DNA base composition is 61 mol % G+C. Formate or hydrogen and carbon dioxide are growth substrates. Sodium chloride is required for growth of both methanogens.

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Abbreviations

SDS:

sodium dodecylsulfate

PIPES:

piperazine-N,N′-bis (2 ethanesulfonic acid)

HS-CoM:

coenzyme M, 2-mercaptoethanesulfonic acid

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Romesser, J.A., Wolfe, R.S., Mayer, F. et al. Methanogenium, a new genus of marine methanogenic bacteria, and characterization ofMethanogenium cariaci sp. nov. andMethanogenium marisnigri sp. nov.. Arch. Microbiol. 121, 147–153 (1979). https://doi.org/10.1007/BF00689979

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  • DOI: https://doi.org/10.1007/BF00689979

Key words

  • Methanogenium cariaci
  • Methanogenium marisnigri
  • Marine methanogenic bacteria
  • Ultrastructure
  • TaxonomyMethanogenium gen. nov.