Archives of Microbiology

, Volume 121, Issue 2, pp 117–120 | Cite as

Glutamine synthetase activity, ammonia assimilation and control of nitrate reduction in the unicellular red algaCyanidium caldarium

  • Carmelo Rigano
  • Vittoria Di Martino Rigano
  • Vincenza Vona
  • Amodio Fuggi
Article

Abstract

Addition ofl-methionine-dl-sulphoximine to cells ofCyanidium caldarium brings about a loss of glutamine synthetase activity. Concomitantly ammonia assimilation is prevented.

Under physiological conditions nitrate reductase [NAD(P)H: nitrate oxidoreductase EC 1.6.6.2] is reversibly converted into an inactive enzyme upon addition of ammonia. In the presence of methionine sulphoximine, when glutamine synthetase activity is lost, nitrate reductase is no longer inactivated by ammonia. It is suggested that ammonia itself is not the actual effector of nitrate reductase inactivation.

Concomitantly with the failure of nitrate reductase to undergo ammonia-inactivation, in the presence of methionine sulphoximine nitrate reduction is an uncontrolled process, thus, in media with nitrate ammonia continues to be produced and excreted into the external medium at a constant rate.

Key words

Cyanidium Red algae Nitrate reductase control 

Abbreviations

NR

Nitrate reductase

GS

Glutamine synthetase

GOGAT

Glutamate syntase

MSX

l-methionine-dl-sulphoximine

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Carmelo Rigano
    • 1
  • Vittoria Di Martino Rigano
    • 1
  • Vincenza Vona
    • 1
  • Amodio Fuggi
    • 1
  1. 1.Istituto di Botanica dell'Università di NapoliNapoliItalia

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